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Sphingomyelin protects against apoptosis and hyperproliferation induced by deoxycholate: potential implications for colon cancer.
- Source :
-
Digestive diseases and sciences [Dig Dis Sci] 2003 Jun; Vol. 48 (6), pp. 1094-101. - Publication Year :
- 2003
-
Abstract
- High fecal deoxycholate levels may promote colonic cancer. Phospholipids protect against bile salt-induced cytotoxicity. We therefore aimed to examine whether the dietary phospholipid sphingomyelin could decrease hyperproliferation induced by deoxycholate. In CaCo2 cells, hyperproliferation (by bromodeoxyuridine assay), phosphorylation state of cellular proteins, and apoptosis with concomitant caspase-3 activity were evaluated after incubation with 50-500 microM deoxycholate, with or without sphingomyelin. At 2 and 4 hr of incubation, deoxycholate induced dose-dependent apoptosis, with concomitant caspase-3 activation. At 16 hr, apoptosis had decreased markedly, but there was dose-dependent hyperproliferation (with changed phosphorylation status of cellular proteins) at this time point. Sphingomyelin dose-dependently reduced deoxycholate-induced apoptosis and hyperproliferation. In conclusion, sphingomyelin reduces deoxycholate-induced hyperproliferation and apoptosis. These findings may have implications for colonic cancer prevention by dietary modification.
- Subjects :
- Caco-2 Cells drug effects
Caspase 3
Caspases metabolism
Colonic Neoplasms prevention & control
Deoxycholic Acid administration & dosage
Dose-Response Relationship, Drug
Enzyme Precursors metabolism
Humans
Sphingomyelins administration & dosage
Time Factors
Apoptosis
Cell Division drug effects
Deoxycholic Acid pharmacology
Sphingomyelins pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0163-2116
- Volume :
- 48
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Digestive diseases and sciences
- Publication Type :
- Academic Journal
- Accession number :
- 12822868
- Full Text :
- https://doi.org/10.1023/a:1023712712025