Back to Search
Start Over
Copper-homocysteine complexes and potential physiological actions.
- Source :
-
Journal of inorganic biochemistry [J Inorg Biochem] 2003 Jul 01; Vol. 95 (4), pp. 321-33. - Publication Year :
- 2003
-
Abstract
- During the last 2 decades it was proposed that atherogenesis was closely related to the homeostasis of homocysteine (hCys) and/or copper. We hypothesized that the physiological action of hCys may be connected with its ability to form complexes with Cu. Our results showed the presence of two different Cu-hCys complexes. At a molar ratio Cu:hCys 1:1, a blue complex most probably consistent with a tentative dimeric Cu(II)(2)(hCys)(2)(H(2)O)(2) formula was formed, with tetrahedral Cu coordination and anti-ferromagnetic properties. The redox processes between Cu(II) and hCys, in a molar ratio > or =1:3 led to formation of a second yellow Cu(I)hCys complex. Both Cu-hCys complexes affected the metabolism of extracellular thiols more than hCys alone and inhibited glutathione peroxidase-1 activity and mRNA abundance. The biological action of hCys and Cu-hCys complexes involved remodeling and phosphorylation of focal adhesion complexes and paxillin. The adhesive interactions of monocytes with an endothelial monolayer led to the redistribution of both paxillin and F-actin after all treatments, but the diapedesis of monocytes through endothelial cell monolayer was both greater and faster in the presence of the tentative Cu(II)(2)(hCys)(2)(H(2)O)(2) complex. Together, these observations suggest that Cu-hCys complexes actively participate in the biochemical responses of endothelial cells that are involved in the aethiopathogenesis of atherosclerosis.
- Subjects :
- Calorimetry
Cell Adhesion drug effects
Cell Line
Cell Survival drug effects
Electron Spin Resonance Spectroscopy
Endothelium cytology
Endothelium drug effects
Endothelium enzymology
Endothelium metabolism
Focal Adhesions drug effects
Glutathione Peroxidase metabolism
Humans
Magnetic Resonance Spectroscopy
Magnetics
Molecular Structure
Monocytes cytology
Monocytes drug effects
Organometallic Compounds chemistry
Organometallic Compounds pharmacology
Phosphorylation drug effects
Phosphotyrosine metabolism
Spectrophotometry, Infrared
Copper metabolism
Copper pharmacology
Homocysteine metabolism
Homocysteine pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0162-0134
- Volume :
- 95
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Journal of inorganic biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 12818803
- Full Text :
- https://doi.org/10.1016/s0162-0134(03)00133-8