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[Structural and functional analysis of the human ribosomal protein L11 gene].
- Source :
-
Molekuliarnaia biologiia [Mol Biol (Mosk)] 2003 May-Jun; Vol. 37 (3), pp. 425-35. - Publication Year :
- 2003
-
Abstract
- The nucleotide sequence was established for the human ribosomal protein L11 gene (HRPL11). The gene (4583 bp) proved to consist of six exons and five introns. The structure of the HRPL11 promoter was shown to be typical for ribosomal protein genes of higher eukaryotes: the promoter lacks TATA or CAAT boxes and has a TATA-like element (ATAA, -24 ... -27) surrounded by GC-rich regions, the transcription start point is within an oligopyrimidine tract, and the 5'-untranslater region is short (26 bp). Proteins interacting with the HRPL11 promoter were identified by the electrophoretic mobility shift assay with oligonucleotide competitors containing known transcription factor-binding sites. The promoter regions were compared for the human genes for ribosomal proteins L11, L32, and S26.
- Subjects :
- 5' Untranslated Regions
Base Sequence
Binding Sites
Cell Nucleus genetics
Cell Nucleus metabolism
Electrophoretic Mobility Shift Assay
GC Rich Sequence
HeLa Cells
Humans
Introns
Molecular Sequence Data
Promoter Regions, Genetic
Ribosomal Proteins metabolism
Sequence Homology, Nucleic Acid
Transcription Factors genetics
Transcription Factors metabolism
Transcription Initiation Site
Aniline Compounds
Ribosomal Proteins genetics
Subjects
Details
- Language :
- Russian
- ISSN :
- 0026-8984
- Volume :
- 37
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Molekuliarnaia biologiia
- Publication Type :
- Academic Journal
- Accession number :
- 12815950