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Gene delivery into prostate cancer cells by holmium laser application.
- Source :
-
Prostate cancer and prostatic diseases [Prostate Cancer Prostatic Dis] 2003; Vol. 6 (2), pp. 127-30. - Publication Year :
- 2003
-
Abstract
- New approaches to treat prostate cancer (PCA) are utilizing gene therapy and aim to correct the disease at the genetic level. Getting a gene efficiently into the target cell is the subject of much interest. We used a holmium laser for transfecting rat PCA cells with the reporter gene pEGFP. By FACS analysis and fluorescence microscopy, we could demonstrate that cellular delivery of plasmid DNA was possible with high efficiencies up to 41.3%. Therefore, transfection of PCA cells by holmium laser might offer a promising new gene transfer strategy to PCA with minimal invasiveness.
- Subjects :
- Adenocarcinoma pathology
Animals
Male
Microscopy, Fluorescence
Plasmids genetics
Prostatic Neoplasms pathology
Rats
Tumor Cells, Cultured
Adenocarcinoma genetics
Adenocarcinoma therapy
Genetic Therapy methods
Holmium therapeutic use
Lasers
Prostatic Neoplasms genetics
Prostatic Neoplasms therapy
Transfection methods
Subjects
Details
- Language :
- English
- ISSN :
- 1365-7852
- Volume :
- 6
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Prostate cancer and prostatic diseases
- Publication Type :
- Academic Journal
- Accession number :
- 12806370
- Full Text :
- https://doi.org/10.1038/sj.pcan.4500653