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Delayed neuronal damage related to microglia proliferation after mild spinal cord compression injury.
Delayed neuronal damage related to microglia proliferation after mild spinal cord compression injury.
- Source :
-
Neuroscience research [Neurosci Res] 2003 Jul; Vol. 46 (3), pp. 309-18. - Publication Year :
- 2003
-
Abstract
- In order to investigate the mechanism of delayed progressive or secondary neuronal damage after the spinal cord injury, we developed a mild-compression injury model in the rat thoracic spinal cord. Our compression device consists of a soft silicone point of contact to the dura, in order to prevent violent injury that may cause axonal tears or hemorrhages in the spinal cord. Since rats often assume a 'standing' posture, i.e. raising head with lifting their fore-limbs, damage to the thoracic spinal cord was evaluated by measuring the frequency of 'standing', which effectively indicates hind limb function. Twenty-four hours after compression by a 20 g weight for 10 or 20 min, the standing frequency of the injured rat was almost the same as that of sham animals that underwent laminectomy without compression. However, the standing frequency decreased with time; the frequency of standing at 72 h was approximately 30-50% that of sham animals. In the compressed spinal cord tissue, microglial cells, detected by lectin staining, proliferated with time. An enormous amount of microglia was observed at 48 and 72 h after compression, although only a small amount of cells were positive to lectin staining at 24 h after the compression. These results suggest that our mild-compression spinal cord injury model showed late-onset or delayed neuronal damage that may be related to pathological microglia proliferation.
- Subjects :
- Animals
Basigin
Behavior, Animal
Cell Count
Disease Models, Animal
Female
In Situ Nick-End Labeling
Lectins metabolism
Membrane Glycoproteins metabolism
Microglia metabolism
Microtubule-Associated Proteins metabolism
Movement
Nerve Degeneration pathology
Nitric Oxide Synthase metabolism
Nitric Oxide Synthase Type II
Posture
Rats
Spinal Cord Compression pathology
Time Factors
Tumor Necrosis Factor-alpha metabolism
Antigens, CD
Antigens, Neoplasm
Antigens, Surface
Avian Proteins
Blood Proteins
Microglia pathology
Nerve Degeneration etiology
Spinal Cord Compression physiopathology
Subjects
Details
- Language :
- English
- ISSN :
- 0168-0102
- Volume :
- 46
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Neuroscience research
- Publication Type :
- Academic Journal
- Accession number :
- 12804792
- Full Text :
- https://doi.org/10.1016/s0168-0102(03)00095-6