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Requirement of transcription factor NFAT in developing atrial myocardium.
- Source :
-
The Journal of cell biology [J Cell Biol] 2003 Jun 09; Vol. 161 (5), pp. 861-74. - Publication Year :
- 2003
-
Abstract
- Nuclear factor of activated T cell (NFAT) is a ubiquitous regulator involved in multiple biological processes. Here, we demonstrate that NFAT is temporally required in the developing atrial myocardium between embryonic day 14 and P0 (birth). Inhibition of NFAT activity by conditional expression of dominant-negative NFAT causes thinning of the atrial myocardium. The thin myocardium exhibits severe sarcomere disorganization and reduced expression of cardiac troponin-I (cTnI) and cardiac troponin-T (cTnT). Promoter analysis indicates that NFAT binds to and regulates transcription of the cTnI and the cTnT genes. Thus, regulation of cytoskeletal protein gene expression by NFAT may be important for the structural architecture of the developing atrial myocardium.
- Subjects :
- Active Transport, Cell Nucleus genetics
Animals
Animals, Newborn
Binding Sites genetics
Cell Nucleus genetics
DNA-Binding Proteins genetics
Down-Regulation genetics
Fetus
Genes, Regulator genetics
Heart Atria metabolism
Mice
Mice, Transgenic
Microscopy, Electron
Mutation genetics
Myocytes, Cardiac metabolism
Myocytes, Cardiac pathology
Myocytes, Cardiac ultrastructure
NFATC Transcription Factors
Promoter Regions, Genetic genetics
Sarcomeres metabolism
Sarcomeres pathology
Sarcomeres ultrastructure
Transcription Factors genetics
Troponin I biosynthesis
Troponin I genetics
Troponin T biosynthesis
Troponin T genetics
DNA-Binding Proteins metabolism
Gene Expression Regulation, Developmental genetics
Heart Atria abnormalities
Heart Atria growth & development
Myocardium metabolism
Nuclear Proteins
Transcription Factors metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9525
- Volume :
- 161
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- The Journal of cell biology
- Publication Type :
- Academic Journal
- Accession number :
- 12796475
- Full Text :
- https://doi.org/10.1083/jcb.200301058