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Effect of hepatocyte growth factor on invasion of prostate cancer cell lines.
- Source :
-
Oncology reports [Oncol Rep] 2003 Jul-Aug; Vol. 10 (4), pp. 1001-6. - Publication Year :
- 2003
-
Abstract
- Hepatocyte growth factor (HGF) was suggested to play an important role in the regulation of mitogenesis, motogenesis, angiogenesis, migration and invasion for various types of cells, and acts through a specific membrane receptor encoded by c-met proto-oncogene. However, the mechanism of the effect of HGF on tumor invasion of prostate cancer cells remains unclear. We investigated the effect of HGF on the invasion of PC-3 and DU-145 prostate cancer cells through a reconstituted basement membrane (Matrigel), the haptotactic migration to fibronectin substrate, the expression of protein and mRNA for matrix metalloproteinases (MMP)-1 and -9, membrane-type 1-MMP (MT1-MMP), urokinase-type plasminogen activator (u-PA) and its receptor (uPAR). HGF increased both Matrigel invasion and haptotactic migration of prostate cancer cells. Furthermore, HGF also increased the production of MMP-1 and -9, MT1-MMP, u-PA and uPAR of these cells. These results suggested that HGF increased the invasive potential of prostate cancer cells probably through enhancement of cell motility and the production of MMPs and u-PA.
- Subjects :
- Antineoplastic Agents metabolism
Cell Adhesion drug effects
Cell Movement drug effects
Collagen
Drug Combinations
Fibrin metabolism
Humans
Laminin
Male
Matrix Metalloproteinase 1 genetics
Matrix Metalloproteinase 1 metabolism
Matrix Metalloproteinase 9 genetics
Matrix Metalloproteinase 9 metabolism
Neoplasm Invasiveness
Prostatic Neoplasms enzymology
Proteoglycans
Proto-Oncogene Mas
RNA, Messenger metabolism
RNA, Neoplasm genetics
Receptors, Cell Surface genetics
Receptors, Cell Surface metabolism
Receptors, Urokinase Plasminogen Activator
Recombinant Proteins pharmacology
Reverse Transcriptase Polymerase Chain Reaction
Tissue Inhibitor of Metalloproteinase-1 genetics
Tissue Inhibitor of Metalloproteinase-1 metabolism
Tumor Cells, Cultured
Urokinase-Type Plasminogen Activator genetics
Urokinase-Type Plasminogen Activator metabolism
Hepatocyte Growth Factor pharmacology
Prostatic Neoplasms pathology
Subjects
Details
- Language :
- English
- ISSN :
- 1021-335X
- Volume :
- 10
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Oncology reports
- Publication Type :
- Academic Journal
- Accession number :
- 12792760