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Interleukin-18 induces expression and release of cytokines from murine glial cells: interactions with interleukin-1 beta.
- Source :
-
Journal of neurochemistry [J Neurochem] 2003 Jun; Vol. 85 (6), pp. 1412-20. - Publication Year :
- 2003
-
Abstract
- Interleukin (IL)-18, a member of the IL-1 cytokine family, is an important mediator of peripheral inflammation and host defence responses. IL-1 is a key proinflammatory cytokine in the brain, but the role of IL-18 in the CNS is not yet clear. The objective of this study was to investigate the actions of IL-18 on mouse glial cells. IL-18 induced intracellular expression of IL-1 alpha and proIL-1 beta, and release of IL-6 from mixed glia. Treatment of lipopolysaccharide-primed microglia with adenosine triphosphate (ATP), an endogenous secondary stimulus, induced IL-1 beta and IL-18 release. Although deletion of the IL-18 gene did not affect IL-1 beta expression or release in this experimental paradigm, IL-1 beta knockout microglia released significantly less IL-18 compared to wild-type microglia. In addition, ATP induced release of mature IL-1 beta from IL-18-primed microglia. These data suggest that IL-18 may contribute to inflammatory responses in the brain, and demonstrate that, in spite of several common features, IL-18 and IL-1 beta differ in their regulation and actions.
- Subjects :
- Adenosine Triphosphate pharmacology
Animals
Cells, Cultured
Drug Interactions
Gene Expression drug effects
Interleukin-1 genetics
Interleukin-1 metabolism
Interleukin-18 genetics
Interleukin-18 metabolism
Interleukin-18 Receptor alpha Subunit
Lipopolysaccharides pharmacology
Mice
Mice, Inbred C57BL
Mice, Knockout
Microglia drug effects
Microglia metabolism
Receptors, Interleukin biosynthesis
Receptors, Interleukin-18
Cytokines metabolism
Interleukin-1 pharmacology
Interleukin-18 pharmacology
Neuroglia drug effects
Neuroglia metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0022-3042
- Volume :
- 85
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Journal of neurochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 12787061
- Full Text :
- https://doi.org/10.1046/j.1471-4159.2003.01787.x