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Use of Spring beauty latent virus to identify compatible interactions between bromovirus components required for virus infection.
- Source :
-
The Journal of general virology [J Gen Virol] 2003 Jun; Vol. 84 (Pt 6), pp. 1367-1375. - Publication Year :
- 2003
-
Abstract
- Spring beauty latent virus (SBLV) is a member of the genus Bromovirus, and is closely related to Brome mosaic virus (BMV) and Cowpea chlorotic mottle virus (CCMV). Compatible interactions between viral components are required for successful infection of plants by BMV and CCMV. To further our understanding of interactions between bromovirus components, we used SBLV to produce reassortants among the three bromoviruses. We found that SBLV RNA 2 functioned with heterologous bromovirus RNA 1 in infections of whole plants and protoplasts of Nicotiana benthamiana, although SBLV RNA 1 did not function with heterologous bromovirus RNA 2. A DNA-based transient assay for 1a and 2a proteins, which are encoded by RNAs 1 and 2, respectively further suggested that SBLV 2a protein may function in combination with heterologous bromovirus 1a protein. Moreover, analysis of the ability of reassortants to spread locally revealed that an RNA 2-mediated interaction between viral components may be required for efficient cell-to-cell movement of bromoviruses.
- Subjects :
- Base Sequence
Bromovirus genetics
DNA, Viral genetics
Movement
Plant Diseases etiology
Plant Diseases virology
Protoplasts virology
RNA, Viral genetics
RNA, Viral metabolism
Nicotiana virology
Viral Proteins genetics
Viral Proteins physiology
Virus Replication
Bromovirus pathogenicity
Bromovirus physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1317
- Volume :
- 84
- Issue :
- Pt 6
- Database :
- MEDLINE
- Journal :
- The Journal of general virology
- Publication Type :
- Academic Journal
- Accession number :
- 12771403
- Full Text :
- https://doi.org/10.1099/vir.0.19020-0