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Genetic analysis of steroid-induced osteonecrosis of the femoral head.
- Source :
-
Journal of orthopaedic science : official journal of the Japanese Orthopaedic Association [J Orthop Sci] 2003; Vol. 8 (3), pp. 329-33. - Publication Year :
- 2003
-
Abstract
- With advanced organ transplantation technology, steroid-induced osteonecrosis of the femoral head (ONF) is one of the most troublesome complications. Steroid sensitivity varies among individuals, and the involvement of polymorphism of various genes relating to steroid metabolism is suggested. The present study investigated the relation between single nucleotide polymorphism (SNP) on the DNA sequence of cytochrome p(450) and ONF development. The subjects were 80 renal transplant patients. Genome DNA was obtained from the peripheral blood, and SNP analyses for CYP3A4, CYP2D6, and CYP2C19 were conducted using various methods: direct sequencing, polymerase chain reaction restriction fragment length polymorphism, and DNA Chip. The relation between ONF development and SNP was statistically analyzed. It is useful if the generating risk judged according to SNPs to prevention of steroid-induced ONF is possible. This time, SNPs, which are clearly related to ONF, were not accepted, although the possibility that SNP related to steroid metabolism is involved in ONF development is important. We think that it is necessary to examine this area more closely.
- Subjects :
- Aryl Hydrocarbon Hydroxylases genetics
Cytochrome P-450 CYP2C19
Cytochrome P-450 CYP2D6 genetics
Cytochrome P-450 CYP3A
Femur Head Necrosis genetics
Glucocorticoids adverse effects
Glucocorticoids immunology
Humans
Immunosuppressive Agents adverse effects
Kidney Transplantation
Mixed Function Oxygenases genetics
Promoter Regions, Genetic
Cytochrome P-450 Enzyme System genetics
Femur Head Necrosis chemically induced
Polymorphism, Single Nucleotide
Subjects
Details
- Language :
- English
- ISSN :
- 0949-2658
- Volume :
- 8
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Journal of orthopaedic science : official journal of the Japanese Orthopaedic Association
- Publication Type :
- Academic Journal
- Accession number :
- 12768474
- Full Text :
- https://doi.org/10.1007/s10776-003-0646-7