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1-40 Beta-amyloid protein fragment modulates the expression of CD44 and CD71 on the astrocytoma cell line in the presence of IL1beta and TNFalpha.
1-40 Beta-amyloid protein fragment modulates the expression of CD44 and CD71 on the astrocytoma cell line in the presence of IL1beta and TNFalpha.
- Source :
-
Journal of cellular physiology [J Cell Physiol] 2003 Jul; Vol. 196 (1), pp. 190-5. - Publication Year :
- 2003
-
Abstract
- The modulation of CD44, VCAM-1 and CD71 expression was analysed by flow cytometry in the 1321N1 astrocytoma cell line in the presence of interleukin-1beta (IL1beta), tumour necrosis factor-alpha (TNFalpha) and 1-40 or 25-35 beta-amyloid (Abeta) fragments. The percentage of 1321N1 astrocytoma cell line expressing these markers increased significantly after treatment with TNFalpha or IL1beta. The presence of Abeta 1-40 fragment, alone or in combination with IL1beta, induced an increase in the percentage of cells expressing CD44, but not VCAM-1. However, the concomitant presence of Abeta 1-40 fragment and of IL1beta or TNFalpha caused an increase in the percentage of CD71 positive cells. In contrast, the shorter Abeta 25-35 fragment was always inactive. These results indicates that Abeta 1-40 fragment, in association with cytokines, can activate this astrocyte-derived cell line and add further elements in favour of the hypothesis that beta-amyloid can act as immunological mediator.<br /> (Copyright 2003 Wiley-Liss, Inc.)
- Subjects :
- Dose-Response Relationship, Drug
Humans
Receptors, Transferrin
Tumor Cells, Cultured
Vascular Cell Adhesion Molecule-1 metabolism
Amyloid beta-Peptides pharmacology
Antigens, CD metabolism
Antigens, Differentiation, B-Lymphocyte metabolism
Astrocytoma metabolism
Gene Expression Regulation, Neoplastic drug effects
Hyaluronan Receptors metabolism
Interleukin-1 pharmacology
Peptide Fragments pharmacology
Tumor Necrosis Factor-alpha pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9541
- Volume :
- 196
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of cellular physiology
- Publication Type :
- Academic Journal
- Accession number :
- 12767055
- Full Text :
- https://doi.org/10.1002/jcp.10295