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Caenorhabditis elegans early embryogenesis and vulval morphogenesis require chondroitin biosynthesis.
- Source :
-
Nature [Nature] 2003 May 22; Vol. 423 (6938), pp. 439-43. - Publication Year :
- 2003
-
Abstract
- Defects in glycosaminoglycan biosynthesis disrupt animal development and can cause human disease. So far much of the focus on glycosaminoglycans has been on heparan sulphate. Mutations in eight squashed vulva (sqv) genes in Caenorhabditis elegans cause defects in cytokinesis during embryogenesis and in vulval morphogenesis during postembryonic development. Seven of the eight sqv genes have been shown to control the biosynthesis of the glycosaminoglycans chondroitin and heparan sulphate. Here we present the molecular identification and characterization of the eighth gene, sqv-5. This gene encodes a bifunctional glycosyltransferase that is probably localized to the Golgi apparatus and is responsible for the biosynthesis of chondroitin but not heparan sulphate. Our findings show that chondroitin is crucial for both cytokinesis and morphogenesis during C. elegans development.
- Subjects :
- Alleles
Amino Acid Sequence
Animals
Caenorhabditis elegans enzymology
Caenorhabditis elegans genetics
Caenorhabditis elegans Proteins analysis
Caenorhabditis elegans Proteins chemistry
Caenorhabditis elegans Proteins genetics
Caenorhabditis elegans Proteins metabolism
Cloning, Molecular
Female
Gene Deletion
Gene Expression Profiling
Glycosyltransferases analysis
Glycosyltransferases chemistry
Glycosyltransferases genetics
Glycosyltransferases metabolism
Heparitin Sulfate biosynthesis
Molecular Sequence Data
Sequence Alignment
Substrate Specificity
Vulva enzymology
Caenorhabditis elegans embryology
Caenorhabditis elegans metabolism
Chondroitin biosynthesis
Morphogenesis
Vulva embryology
Vulva metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0028-0836
- Volume :
- 423
- Issue :
- 6938
- Database :
- MEDLINE
- Journal :
- Nature
- Publication Type :
- Academic Journal
- Accession number :
- 12761549
- Full Text :
- https://doi.org/10.1038/nature01634