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Effect of docosahexaenoic acid and ascorbate on peroxidation of retinal membranes of ODS rats.
- Source :
-
Free radical research [Free Radic Res] 2003 Apr; Vol. 37 (4), pp. 419-24. - Publication Year :
- 2003
-
Abstract
- Mutant male osteogenic disorder Shionogi (ODS) rats, unable to synthesize ascorbic acid, were fed diets containing a high content of docosahexaenoic acid (DHA) and different amounts of ascorbic acid, to study the effect of DHA on peroxidative susceptibility of the retina and possible antioxidant action of ascorbic acid. ODS rats were fed from 7 weeks of age with diets containing high DHA (6.4% of total energy). A control group received a diet high in linoleic acid. The diets also contained varying amounts of ascorbic acid. Fatty acid compositions and phospholipid hydroperoxides in rod outer segment (ROS) membranes, and retinal ascorbic acid were analyzed. DHA in ROS membranes was significantly increased in rats fed high DHA, compared with the linoleic acid diet. Levels of phospholipid hydroperoxides in the DHA-fed rats were significantly higher than the linoleic acid-fed rats. Ascorbic acid supplementation did not suppress the phospholipid hydroperoxide levels after a high DHA diet, even when the supplement increased the content of retinal ascorbic acid. In conclusion, high DHA feeding induced a marked increase of phospholipid hydroperoxides in ROS membranes of ODS rats. Supplementation of ascorbic acid did not reverse this increase.
- Subjects :
- Animals
Antioxidants metabolism
Ascorbic Acid metabolism
Chromatography, High Pressure Liquid
Fatty Acids metabolism
Hydrogen Peroxide metabolism
Linoleic Acid pharmacology
Peroxides metabolism
Phosphatidylcholines metabolism
Rats
Rod Cell Outer Segment drug effects
Time Factors
Ascorbic Acid pharmacology
Cell Membrane metabolism
Docosahexaenoic Acids pharmacology
Retina drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1071-5762
- Volume :
- 37
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Free radical research
- Publication Type :
- Academic Journal
- Accession number :
- 12747736
- Full Text :
- https://doi.org/10.1080/1071576031000070084