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STA11, a Chlamydomonas reinhardtii locus required for normal starch granule biogenesis, encodes disproportionating enzyme. Further evidence for a function of alpha-1,4 glucanotransferases during starch granule biosynthesis in green algae.
- Source :
-
Plant physiology [Plant Physiol] 2003 May; Vol. 132 (1), pp. 137-45. - Publication Year :
- 2003
-
Abstract
- In Chlamydomonas reinhardtii, the presence of a defective STA11 locus results in significantly reduced granular starch deposition displaying major modifications in shape and structure. This defect simultaneously leads to the accumulation of linear malto-oligosaccharides (MOS). The mutants of STA11 were showed to lack D-enzyme, a plant alpha-1,4 glucanotransferase analogous to the Escherichia coli amylomaltase. We have cloned and characterized both the cDNA and gDNA corresponding to the C. reinhardtii D-enzyme. We now report allele-specific modifications of the D-enzyme gene in the mutants of STA11. These allele-specific modifications cosegregate with the corresponding sta11 mutations, thereby demonstrating that STA11 encodes D-enzyme. MOS production and starch accumulation were investigated during day and night cycles in wild-type and mutant C. reinhardtii cells. We demonstrate that in the algae MOS are produced during starch biosynthesis and degraded during the phases of net polysaccharide catabolism.
- Subjects :
- Algal Proteins genetics
Algal Proteins metabolism
Alleles
Amino Acid Sequence
Animals
Chlamydomonas reinhardtii enzymology
Cloning, Molecular
DNA chemistry
DNA genetics
DNA, Complementary chemistry
DNA, Complementary genetics
Glycogen Debranching Enzyme System metabolism
Molecular Sequence Data
Mutation
Oligosaccharides metabolism
Phylogeny
Polymorphism, Restriction Fragment Length
RNA, Messenger genetics
RNA, Messenger metabolism
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Starch metabolism
Chlamydomonas reinhardtii genetics
Glycogen Debranching Enzyme System genetics
Starch biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 0032-0889
- Volume :
- 132
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Plant physiology
- Publication Type :
- Academic Journal
- Accession number :
- 12746519
- Full Text :
- https://doi.org/10.1104/pp.102.016527