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Single-cell microarray analysis in hippocampus CA1: demonstration and validation of cellular heterogeneity.
- Source :
-
The Journal of neuroscience : the official journal of the Society for Neuroscience [J Neurosci] 2003 May 01; Vol. 23 (9), pp. 3607-15. - Publication Year :
- 2003
-
Abstract
- Laser capture microdissection in combination with microarrays allows for the expression analysis of thousands of genes in selected cells. Here we describe single-cell gene expression profiling of CA1 neurons in the rat hippocampus using a combination of laser capture, T7 RNA amplification, and cDNA microarray analysis. Subsequent cluster analysis of the microarray data identified two different cell types: pyramidal neurons and an interneuron. Cluster analysis also revealed differences among the pyramidal neurons, indicating that even a single cell type in vivo is not a homogeneous population of cells at the gene expression level. Microarray data were confirmed by quantitative RT-PCR and in situ hybridization. We also report on the reproducibility and sensitivity of this combination of methods. Single-cell gene expression profiling offers a powerful tool to tackle the complexity of the mammalian brain.
- Subjects :
- Animals
Bacteriophage T7 genetics
Cell Count
Cluster Analysis
Female
Hippocampus cytology
In Situ Hybridization
Interneurons chemistry
Interneurons metabolism
Lasers
Neurons chemistry
Pyramidal Cells chemistry
Pyramidal Cells metabolism
RNA, Antisense analysis
RNA, Antisense genetics
RNA, Messenger analysis
RNA, Messenger metabolism
Rats
Reproducibility of Results
Reverse Transcriptase Polymerase Chain Reaction
Sensitivity and Specificity
Hippocampus metabolism
Neurons metabolism
Oligonucleotide Array Sequence Analysis methods
Subjects
Details
- Language :
- English
- ISSN :
- 1529-2401
- Volume :
- 23
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- The Journal of neuroscience : the official journal of the Society for Neuroscience
- Publication Type :
- Academic Journal
- Accession number :
- 12736331