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Bioenergetic analysis of peroxisome proliferator-activated receptor gamma coactivators 1alpha and 1beta (PGC-1alpha and PGC-1beta) in muscle cells.
- Source :
-
The Journal of biological chemistry [J Biol Chem] 2003 Jul 18; Vol. 278 (29), pp. 26597-603. Date of Electronic Publication: 2003 May 06. - Publication Year :
- 2003
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Abstract
- Peroxisome proliferator-activated receptor gamma coactivator (PGC)-1alpha is a coactivator of nuclear receptors and other transcription factors that regulates several components of energy metabolism, particularly certain aspects of adaptive thermogenesis in brown fat and skeletal muscle, hepatic gluconeogenesis, and fiber type switching in skeletal muscle. PGC-1alpha has been shown to induce mitochondrial biogenesis when expressed in muscle cells, and preliminary analysis has suggested that this molecule may specifically increase the fraction of uncoupled versus coupled respiration. In this paper, we have performed detailed bioenergetic analyses of the function of PGC-1alpha and its homolog PGC-1beta in muscle cells by monitoring simultaneously oxygen consumption and membrane potential. Cells expressing PGC-1alpha or PGC-1beta display higher proton leak rates at any given membrane potential than control cells. However, cells expressing PGC-1alpha have a higher proportion of their mitochondrial respiration linked to proton leak than cells expressing PGC-1beta. Although these two proteins cause a similar increase in the expression of many mitochondrial genes, PGC-1beta preferentially induces certain genes involved in the removal of reactive oxygen species, recently recognized as activators of uncoupling proteins. Together, these data indicate that PGC-1alpha and PGC-1beta profoundly alter mitochondrial metabolism and suggest that these proteins are likely to play different physiological functions.
- Subjects :
- Adenosine Triphosphate metabolism
Animals
Cell Line
Energy Metabolism
Green Fluorescent Proteins
Kinetics
Luminescent Proteins genetics
Luminescent Proteins metabolism
Membrane Potentials
Mice
Mice, Transgenic
Microscopy, Electron
Mitochondria, Muscle metabolism
Muscle, Skeletal cytology
Oxygen Consumption
Protons
Recombinant Proteins genetics
Recombinant Proteins metabolism
Transcription Factors genetics
Muscle, Skeletal metabolism
Receptors, Cytoplasmic and Nuclear metabolism
Transcription Factors metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9258
- Volume :
- 278
- Issue :
- 29
- Database :
- MEDLINE
- Journal :
- The Journal of biological chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 12734177
- Full Text :
- https://doi.org/10.1074/jbc.M301850200