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Modulation of immune system function by measles virus infection. II. Infection of B cells leads to the production of a soluble factor that arrests uninfected B cells in G0/G1.
- Source :
-
Viral immunology [Viral Immunol] 2003; Vol. 16 (1), pp. 45-55. - Publication Year :
- 2003
-
Abstract
- Measles can result in a variety of immunologic defects. Previously we showed that an Epstein-Barr virus-transformed B cell line (B cells), when infected with measles virus, produced a soluble antiproliferative factor that inhibited proliferation of T and B cells. Here we explore the effects of infection by measles virus versus the virus-free soluble antiproliferative factor on B cells. The B cells showed no change in the amounts of interleukin (IL)-2, 10, 12, interferon (IFN)-gamma, or transforming growth factor (TGF)-beta when infected or exposed to the soluble factor. Similarly, B cells showed no change in the expression of class II major histocompatibility antigens, LFA-1, ICAM-1, CD19, CD40, CD80, CD86, CD95 (Fas), or CD178 (FasL). Cell cycle analysis showed that measles virus infection caused an accumulation of cells in S and G(2)/M phases with a "sub-G(1)" cell population, while incubation of cells with the soluble factor caused an accumulation in G(0)/G(1). These experiments provide evidence that measles virus causes a profound inhibition of B cell proliferation without distinguishable changes in cytokine profile or cell surface phenotype. Further, it appears that there are two populations of cells affected by infection: one population is growth arrested due to the influence of the immunosuppressive factor and is not infected; a second population that is infected progresses through S phase less efficiently. Alternatively, while both the soluble factor and live virus infection may affect cells in G(0)/G(1) phases, only live virus infection could selectively induce apoptosis of G(0)/G(1) cells, resulting in cell accumulation in S and G(2)/M phases with a build up of "sub-G(1)" cells.
- Subjects :
- B-Lymphocytes virology
Cell Line, Transformed
Culture Media, Conditioned
Humans
Immunologic Factors metabolism
Lymphocyte Activation
Measles physiopathology
Measles virology
Measles virus immunology
Solubility
B-Lymphocytes immunology
G1 Phase drug effects
Immunologic Factors pharmacology
Measles immunology
Measles virus pathogenicity
Resting Phase, Cell Cycle drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 0882-8245
- Volume :
- 16
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Viral immunology
- Publication Type :
- Academic Journal
- Accession number :
- 12725688
- Full Text :
- https://doi.org/10.1089/088282403763635447