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The regulation of bone resorption in tooth formation and eruption processes in mouse alveolar crest devoid of cathepsin k.
- Source :
-
Journal of pharmacological sciences [J Pharmacol Sci] 2003 Apr; Vol. 91 (4), pp. 285-94. - Publication Year :
- 2003
-
Abstract
- Osteoclastic bone resorption has recently been implicated in the tooth formation and eruption in alveolar bone. Cathepsin K (CK) is a cysteine proteinase expressed predominantly in osteoclasts and is believed to play a critical role in degradation of bone matrix proteins. Here we present evidence that the alveolar bone resorption is essential for the tooth formation and that eruption proceeds normally in CK-deficient (CK-/-) mice. Radiographic and histological analyses revealed that the alveolar bone from these animals had no significant abnormalities during the tooth development between 5 and 28 days after birth. The tooth crown was normally erupted through the alveolar bone layer at 28 days after birth. The number of tartrate-resistant acid phosphatase-positive multinuclear cells in the alveolar bone around the tooth germ was apparently increased in 5-day-old CK-/- mice compared with age-matched littermates. More important, however, the immunohistochemical localization of matrix metalloproteinase-9 (MMP-9) was clearly increased in the CK-/- osteoclasts. In contrast, no significant difference in the immunoreactivity for cathepsin D was observed between the CK-/- osteoclasts and the wild-type ones. These results indicate that CK-/- osteoclasts are fully differentiated and are capable of degrading the organic phase of alveolar bone during the tooth formation and eruption, which may result from the compensatory action by MMP-9 increasingly expressed in the osteoclasts.
- Subjects :
- Alveolar Bone Loss genetics
Alveolar Bone Loss metabolism
Animals
Cathepsin K
Cathepsins physiology
Mice
Mice, Inbred C57BL
Mice, Knockout
Osteoclasts metabolism
Tooth physiology
Tooth Eruption physiology
Bone Resorption genetics
Cathepsins deficiency
Cathepsins genetics
Tooth growth & development
Tooth metabolism
Tooth Eruption genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1347-8613
- Volume :
- 91
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Journal of pharmacological sciences
- Publication Type :
- Academic Journal
- Accession number :
- 12719657
- Full Text :
- https://doi.org/10.1254/jphs.91.285