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PCR method for detection of largemouth bass virus.
- Source :
-
Diseases of aquatic organisms [Dis Aquat Organ] 2003 Mar 17; Vol. 54 (1), pp. 29-33. - Publication Year :
- 2003
-
Abstract
- A polymerase chain reaction (PCR) method was developed for largemouth bass virus (LMBV). This iridovirus can cause a lethal disease of largemouth bass Micropterus salmoides, but also subclinically infects largemouth bass and other species of fishes. Oligonucleotide primers were designed to specifically amplify the major capsid protein gene of LMBV. The protocol for sample processing and PCR provided a method that was more sensitive than cell culture for detection of LMBV in fish. The specific amplification of LMBV also provided an improved method for confirming the identity of cell-culture isolates presumptively identified as LMBV.
- Subjects :
- Animals
Base Sequence
Capsid chemistry
DNA Virus Infections diagnosis
DNA Virus Infections virology
DNA, Viral chemistry
Fish Diseases virology
Gene Amplification
Iridovirus genetics
Molecular Sequence Data
Polymerase Chain Reaction methods
Sensitivity and Specificity
Bass
Capsid Proteins
DNA Virus Infections veterinary
Fish Diseases diagnosis
Iridovirus isolation & purification
Polymerase Chain Reaction veterinary
Subjects
Details
- Language :
- English
- ISSN :
- 0177-5103
- Volume :
- 54
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Diseases of aquatic organisms
- Publication Type :
- Academic Journal
- Accession number :
- 12718467
- Full Text :
- https://doi.org/10.3354/dao054029