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Increased RhoA translocation in renal cortex of diabetic rats.
- Source :
-
Life sciences [Life Sci] 2003 May 16; Vol. 72 (26), pp. 2943-52. - Publication Year :
- 2003
-
Abstract
- RhoA, a member of the Rho small G protein family, mediates multiple intracellular signaling pathways, and is highly expressed in renal cortex. RhoA translocation is associated with RhoA activation. This study was undertaken to examine the relation of translocation of RhoA in the renal cortex with diabetic renal injury in streptozotocin (STZ)-induced diabetic rats. Male Sprague-Dawley rats were divided into control and diabetic groups and were studied at 8 weeks after STZ-injection (55 mg/kg, i.v). We found that the kidney weight and urinary protein excretion were significantly increased in diabetic rats. Diabetic glomerulopathy was confirmed by mesangial matrix expanding and glomerular basement membrane thickening. The ratio of membrane-bound RhoA verses cytosolic RhoA is 1.8 fold higher (p < 0.01) in diabetic group, indicating an enhanced RhoA translocation. There was no significant difference in total RhoA protein expression and RhoA mRNA expression between diabetic and control groups. These data suggest that RhoA translocation might be involved in diabetic renal injury.
- Subjects :
- Animals
Body Weight
Cell Fractionation
Kidney Cortex cytology
Kidney Cortex pathology
Male
Organ Size
Proteinuria metabolism
Rats
Rats, Sprague-Dawley
rhoA GTP-Binding Protein genetics
Diabetes Mellitus, Experimental metabolism
Kidney Cortex metabolism
Protein Transport physiology
rhoA GTP-Binding Protein metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0024-3205
- Volume :
- 72
- Issue :
- 26
- Database :
- MEDLINE
- Journal :
- Life sciences
- Publication Type :
- Academic Journal
- Accession number :
- 12706482
- Full Text :
- https://doi.org/10.1016/s0024-3205(03)00228-5