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Localized proton MRS of cerebral metabolite profiles in different mouse strains.
- Source :
-
Magnetic resonance in medicine [Magn Reson Med] 2003 May; Vol. 49 (5), pp. 822-7. - Publication Year :
- 2003
-
Abstract
- Localized proton MR spectroscopy (MRS) was used to quantify cerebral metabolite concentrations in NMRI (n = 8), BALB/c (n = 7), and C57BL/6 (n = 8) mice in vivo and 1 hr after global irreversible ischemia (2.35 T, STEAM, TR/TE/TM = 6000/20/10 ms, 4 x 3 x 4 mm(3) volume, corrections for cerebrospinal fluid). Anatomical MRI and proton MRS revealed significant differences of the C57BL/6 strain in comparison with both BALB/c and NMRI mice. While MRI volumetry yielded larger ventricular spaces of the C57BL/6 strain, proton MRS resulted in elevated concentrations of N-acetylaspartate (tNAA), creatine and phosphocreatine (tCr), choline-containing compounds (Cho), glucose (Glc), and lactate (Lac) relative to BALB/c mice and elevated Glc relative to NMRI mice. Apart from the expected decrease of Glc and increase of Lac 1 hr post mortem, C57BL/6 mice presented with significant reductions of tNAA, tCr, and Cho, whereas these metabolites remained unchanged in BALB/c and NMRI mice. The results support the hypothesis that the more pronounced vulnerability of C57BL/6 mice to brain ischemia is linked to strain-dependent differences of the cerebral energy metabolism.<br /> (Copyright 2003 Wiley-Liss, Inc.)
Details
- Language :
- English
- ISSN :
- 0740-3194
- Volume :
- 49
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Magnetic resonance in medicine
- Publication Type :
- Academic Journal
- Accession number :
- 12704764
- Full Text :
- https://doi.org/10.1002/mrm.10445