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Analysis of 4.3 kilobases of divergent locus B of macaque retroperitoneal fibromatosis-associated herpesvirus reveals a close similarity in gene sequence and genome organization to Kaposi's sarcoma-associated herpesvirus.
- Source :
-
Journal of virology [J Virol] 2003 May; Vol. 77 (9), pp. 5084-97. - Publication Year :
- 2003
-
Abstract
- We previously identified retroperitoneal fibromatosis-associated herpesvirus (RFHV) as a simian homolog of Kaposi's sarcoma-associated herpesvirus (KSHV) in a fibroproliferative malignancy of macaques that has similarities to Kaposi's sarcoma. In this report, we cloned 4.3 kb of divergent locus B (DL-B) flanking the DNA polymerase gene from two variants of RFHV from different species of macaque with a consensus degenerate hybrid oligonucleotide primer approach. Within the DL-B region of RFHV, viral homologs of the cellular interleukin-6, dihydrofolate reductase, and thymidylate synthase genes were identified, along with a homolog of the gammaherpesvirus open reading frame (ORF) 10. In addition, a homolog of the KSHV ORF K3, the modulator of immune recognition-1, was identified. Our data show a close similarity in sequence conservation, gene content, and genomic structure between RFHV and KSHV which strongly supports the grouping of these viral species within the same RV-1 rhadinovirus lineage and the hypothesis that RFHV is the macaque homolog of KSHV.
- Subjects :
- Amino Acid Sequence
Animals
Base Sequence
Cloning, Molecular
DNA-Directed DNA Polymerase genetics
Herpesviridae Infections veterinary
Herpesviridae Infections virology
Herpesvirus 8, Human chemistry
Humans
Macaca mulatta
Macaca nemestrina
Molecular Sequence Data
Monkey Diseases virology
Phylogeny
Retroperitoneal Fibrosis veterinary
Retroperitoneal Fibrosis virology
Retroperitoneal Neoplasms veterinary
Retroperitoneal Neoplasms virology
Sarcoma, Kaposi virology
Sequence Analysis, DNA
Tumor Virus Infections veterinary
Tumor Virus Infections virology
Evolution, Molecular
Herpesvirus 8, Human classification
Herpesvirus 8, Human genetics
Rhadinovirus chemistry
Rhadinovirus classification
Rhadinovirus genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0022-538X
- Volume :
- 77
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Journal of virology
- Publication Type :
- Academic Journal
- Accession number :
- 12692211
- Full Text :
- https://doi.org/10.1128/jvi.77.9.5084-5097.2003