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Neg, a nerve growth factor-stimulated gene expressed by fetal neocortical neurons that is downregulated by ethanol.
- Source :
-
The Journal of comparative neurology [J Comp Neurol] 2003 May 26; Vol. 460 (2), pp. 212-22. - Publication Year :
- 2003
-
Abstract
- Neurotrophins are critical for neuronal development, plasticity, and survival. Ethanol affects these processes. We tested the hypothesis that ethanol inhibits nerve growth factor (NGF)-stimulated gene expression. Dissociated cultures of fetal cortical neurons were treated with NGF and/or ethanol. NGF sustained cell viability and reduced the incidence of terminal uridylated nick-end labeling and pyknosis. Ethanol eliminated these effects and induced neuronal death. Differential display of mRNA showed that one gene fragment (245 bp) was expressed by cells treated with NGF alone; ethanol blocked its expression. This fragment, named neg (nerve growth factor-stimulated, ethanol-depressed gene), has high nucleotide identity with genes from human myeloid cells and murine lymphocytes. Ribonuclease protection assay and in situ hybridization verified NGF upregulation and ethanol antagonism. Thus, ethanol specifically alters the expression of a gene that appears to be involved in NGF-mediated neuroprotection.<br /> (Copyright 2003 Wiley-Liss, Inc.)
- Subjects :
- Amino Acid Sequence drug effects
Amino Acid Sequence physiology
Animals
Base Sequence drug effects
Base Sequence physiology
Down-Regulation drug effects
Down-Regulation physiology
Female
Fetus embryology
Fetus metabolism
Gene Expression Regulation, Developmental drug effects
Gene Expression Regulation, Developmental physiology
Molecular Sequence Data
Neocortex embryology
Neocortex metabolism
Neurons metabolism
Pregnancy
RNA, Messenger biosynthesis
Rats
Rats, Sprague-Dawley
Ethanol pharmacology
Fetus drug effects
Neocortex drug effects
Nerve Growth Factor biosynthesis
Neurons drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9967
- Volume :
- 460
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- The Journal of comparative neurology
- Publication Type :
- Academic Journal
- Accession number :
- 12687686
- Full Text :
- https://doi.org/10.1002/cne.10651