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Suppressive effects of transforming growth factor-beta1 produced by hepatocellular carcinoma cell lines on interferon-gamma production by peripheral blood mononuclear cells.
- Source :
-
Acta medica Okayama [Acta Med Okayama] 2002 Dec; Vol. 56 (6), pp. 309-15. - Publication Year :
- 2002
-
Abstract
- Transforming growth factor-beta1 (TGF-beta1) exerts potent immunosuppressive effects. In this study, we investigated the potential role of TGF-beta1 produced by hepatocellular carcinoma (HCC) cell lines in immunosuppression mechanisms. Using the Mv1Lu cell-growth inhibition assay and an enzyme-linked immunosorbent assay (ELISA), we detected optimal levels of TGF-beta1 in the culture supernatants conditioned by the HCC cell lines PLC/PRF/5, Hep3B, and HepG2. To determine the biological activity of TGF-beta1 in the supernatants, we examined the effects of the culture supernatants on the production of interferon (IFN)-gamma induced during the culture of peripheral blood mononuclear cells (PBMCs) stimulated with interleukin (IL)-12. IFN-gamma production of IL-12-stimulated PBMCs in the 1:1 dilution of the acid-activated conditioned medium of PLC/PRF/5, Hep3B, and HepG2 reduced to 14.7 +/- 0.8, 17.3 +/- 9.0, and 35.9 +/- 14.6%, respectively, compared with the value in the culture with control medium (complete culture medium). These results suggest that HCC cells producing TGF-beta1 may reduce the generation or activation of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells, and thus could enhance their ability to escape immune-mediated surveillance.
- Subjects :
- Aged
Female
Humans
Interferon-gamma biosynthesis
Interleukin-12 pharmacology
Male
Middle Aged
Transforming Growth Factor beta1
Tumor Cells, Cultured
Carcinoma, Hepatocellular immunology
Immune Tolerance
Leukocytes, Mononuclear immunology
Liver Neoplasms immunology
Transforming Growth Factor beta physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0386-300X
- Volume :
- 56
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Acta medica Okayama
- Publication Type :
- Academic Journal
- Accession number :
- 12685860
- Full Text :
- https://doi.org/10.18926/AMO/31688