Back to Search
Start Over
DNA demethylation is directly related to tumour progression: evidence in normal, pre-malignant and malignant cells from uterine cervix samples.
- Source :
-
Oncology reports [Oncol Rep] 2003 May-Jun; Vol. 10 (3), pp. 545-9. - Publication Year :
- 2003
-
Abstract
- A computer-assisted assay based on the quantitative analysis of DNA methylation in individual interphase nuclei by indirect immunolabelling with anti-5-methylcytosine antibodies was recently developed in our laboratory. In situ analyses were performed on individual nuclei from normal and experimentally hypo- or hypermethylated cultured cells as well as on human peripheral blood B-lymphocytes from normal and chronic lymphoid leukemia (CLL) samples. We present the results obtained on cells from patients affected by different degrees of preneoplastic or neoplastic changes of the uterine cervix as compared to normal controls. The analysis of DNA methylation in individual cells from cytofuge samples was performed as follows: within each nucleus the eu- and heterochromatin methylation levels were quantified in the grey scale range by dedicated software in terms of numbers, areas and optical densities (ODs) of the immunolabeled dense heterochromatic regions ("spots"), and of the optical density of nuclear background, i.e., of nuclear euchromatin. Analogously, in randomly chosen microscope fields of tissue sections from paraffin-embedded samples, progressive tissue demethylation was observed in dysplastic and cancer cells as compared to normal ones. Both methods showed significant and progressive DNA hypomethylation in dysplastic and cancer cells as compared to control specimens.
- Subjects :
- 5-Methylcytosine immunology
Adult
Antibodies, Monoclonal analysis
Carcinoma in Situ genetics
Carcinoma in Situ metabolism
Carcinoma in Situ pathology
Carcinoma, Squamous Cell genetics
Carcinoma, Squamous Cell metabolism
Carcinoma, Squamous Cell pathology
Cell Line
Cervix Uteri metabolism
Cervix Uteri pathology
Disease Progression
Epithelial Cells metabolism
Epithelial Cells pathology
Female
Fluorescent Antibody Technique, Indirect
Humans
In Situ Hybridization, Fluorescence
Middle Aged
Precancerous Conditions metabolism
Precancerous Conditions pathology
Uterine Neoplasms metabolism
Uterine Neoplasms pathology
5-Methylcytosine analysis
DNA Methylation
DNA, Neoplasm metabolism
Image Processing, Computer-Assisted methods
Precancerous Conditions genetics
Uterine Neoplasms genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1021-335X
- Volume :
- 10
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Oncology reports
- Publication Type :
- Academic Journal
- Accession number :
- 12684621