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A subset of Toll-like receptor ligands induces cross-presentation by bone marrow-derived dendritic cells.
- Source :
-
Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 2003 Apr 15; Vol. 170 (8), pp. 4102-10. - Publication Year :
- 2003
-
Abstract
- Dendritic cells (DCs) are capable of cross-presenting exogenous Ag to CD8(+) CTLs. Detection of microbial products by Toll-like receptors (TLRs) leads to activation of DCs and subsequent orchestration of an adaptive immune response. We hypothesized that microbial TLR ligands could activate DCs to cross-present Ag to CTLs. Using DCs and CTLs in an in vitro cross-presentation system, we show that a subset of microbial TLR ligands, namely ligands of TLR3 (poly(inosinic-cytidylic) acid) and TLR9 (immunostimulatory CpG DNA), induces cross-presentation. In contrast to presentation of Ag to CD4(+) T cells by immature DCs, TLR-induced cross-presentation is mediated by mature DCs, is independent of endosomal acidification, and relies on cytosolic Ag processing machinery.
- Subjects :
- Adaptor Proteins, Signal Transducing
Adjuvants, Immunologic physiology
Animals
Antigens, Bacterial immunology
Antigens, Bacterial metabolism
Antigens, Differentiation physiology
Bone Marrow Cells cytology
Cell Differentiation immunology
Cells, Cultured
CpG Islands immunology
Cytosol immunology
Cytosol metabolism
Dendritic Cells cytology
Down-Regulation immunology
Flagellin immunology
Flagellin metabolism
Histocompatibility Antigens Class I immunology
Histocompatibility Antigens Class I metabolism
Histocompatibility Antigens Class II immunology
Histocompatibility Antigens Class II metabolism
Intracellular Fluid immunology
Intracellular Fluid metabolism
Ligands
Lipopolysaccharides immunology
Lipopolysaccharides metabolism
Male
Membrane Glycoproteins biosynthesis
Membrane Glycoproteins physiology
Mice
Mice, Inbred C57BL
Mice, Knockout
Mice, Transgenic
Myeloid Differentiation Factor 88
Peptidoglycan immunology
Peptidoglycan metabolism
Poly I-C immunology
Poly I-C metabolism
Receptors, Cell Surface biosynthesis
Receptors, Cell Surface physiology
Receptors, Immunologic physiology
Toll-Like Receptor 3
Toll-Like Receptors
Antigen Presentation immunology
Bone Marrow Cells immunology
Bone Marrow Cells metabolism
Dendritic Cells immunology
Dendritic Cells metabolism
Membrane Glycoproteins metabolism
Receptors, Cell Surface metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1767
- Volume :
- 170
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Journal of immunology (Baltimore, Md. : 1950)
- Publication Type :
- Academic Journal
- Accession number :
- 12682240
- Full Text :
- https://doi.org/10.4049/jimmunol.170.8.4102