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[Preparation and application of monoclonal antibodies to recombinant human IFN alpha].
- Source :
-
Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology [Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi] 2002 Sep; Vol. 16 (3), pp. 261-3. - Publication Year :
- 2002
-
Abstract
- Objective: To prepare and apply monoclonal antibodies (McAb) against recombinant human interferon alpha (rHu IFN-alpha).<br />Methods: Five cell lines (2E9, 4G1, 2A7, 2C9, 4G10) secreting McAbs against rHu IFN-alpha were established by hybridoma technique.<br />Results: All the cell lines secreted monoclonal antibodies stably. Functions of secreting antibodies of the five cell lines lasted for 6 months in BALB/c mice and 8 months in cell culture. The specificity of antibody was constant. The Ig subclasses of the McAbs were IgG1. Anti-IFN McAb affinity purification column was prepared by coupling the anti IFN-alpha McAb to Sepharose 4B. The combining rate reached was higher than 95%.<br />Conclusions: The highest purification efficiency was obtained by using 4G10 column.
Details
- Language :
- Chinese
- ISSN :
- 1003-9279
- Volume :
- 16
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology
- Publication Type :
- Academic Journal
- Accession number :
- 12665935