Preparation of 1,2-3H-21-deoxycortisol by incubation of 1,2-3H-17-hydroxyprogesterone with rat adrenal mitochondria.

Authors :: Maschler I
Horn H
Source :: Steroids [Steroids] 1976 Jan; Vol. 27 (1), pp. 1-3.
Publication Year :: 1976
Abstract: A method is described for preparation of 1,2-3H-21-deoxycortisol by incubation of 1,2-3H-17-hydroxyprogesterone with rat adrenal mitoochondria. The radiochemical purity of the product was established by two subsequent chromatographies, reversed isotopic dilution and oxidation to 21-deoxycortisone. The final yield of 1,2-3H-21-deoxycortisol was approximately 15%.

Subjects :: Animals
Cortodoxone biosynthesis
Cortodoxone isolation & purification
Crystallization
Female
Hydroxylation
In Vitro Techniques
Rats
Tritium
17-Hydroxycorticosteroids analogs & derivatives
Adrenal Glands ultrastructure
Cortodoxone analogs & derivatives
Hydroxyprogesterones metabolism
Mitochondria metabolism

Full Text Access

Tools