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The pleckstrin homology domain of phospholipase C-beta2 as an effector site for Rac.
- Source :
-
The Journal of biological chemistry [J Biol Chem] 2003 Jun 06; Vol. 278 (23), pp. 21099-104. Date of Electronic Publication: 2003 Mar 25. - Publication Year :
- 2003
-
Abstract
- Increasing evidence links the activation of Rho family GTPases to the stimulation of lipid hydrolysis catalyzed by phospholipase C (PLC)-beta isozymes. To better define this relationship, members of a library of recombinant Rho GTPases were screened for their capacity to directly engage various purified PLC-beta isozymes. Of the 17 tested members of the Rho family, only the active isoforms of Rac (Rac1, Rac2, and Rac3) both stimulate PLC-beta activity in vivo and bind PLC-beta2 and PLC-beta3, but not PLC-beta1, in vitro. Furthermore, the recognition site for Rac GTPases was localized to the pleckstrin homology (PH) domain of PLC-beta2, and this PH domain is fully sufficient to selectively interact with the active versions of the Rac GTPases, but not with other similar Rho GTPases. Together, these findings present a quantitative evaluation of the direct interactions between Rac GTPases and PLC-beta isozymes and define a novel role for the PH domain of PLC-beta2 as a putative effector site for Rac GTPases.
- Subjects :
- Animals
Biosensing Techniques
COS Cells
DNA, Complementary
Enzyme Activation
Humans
Isoenzymes metabolism
Phospholipase C beta
Protein Binding
Protein Structure, Tertiary
Type C Phospholipases metabolism
rac GTP-Binding Proteins metabolism
RAC2 GTP-Binding Protein
Blood Proteins genetics
Isoenzymes chemistry
Isoenzymes genetics
Phosphoproteins genetics
Type C Phospholipases chemistry
Type C Phospholipases genetics
rac1 GTP-Binding Protein metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9258
- Volume :
- 278
- Issue :
- 23
- Database :
- MEDLINE
- Journal :
- The Journal of biological chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 12657629
- Full Text :
- https://doi.org/10.1074/jbc.M301418200