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Bacillus subtilis ResA is a thiol-disulfide oxidoreductase involved in cytochrome c synthesis.
- Source :
-
The Journal of biological chemistry [J Biol Chem] 2003 May 16; Vol. 278 (20), pp. 17852-8. Date of Electronic Publication: 2003 Mar 07. - Publication Year :
- 2003
-
Abstract
- Covalent attachment of heme to apocytochromes c in bacteria occurs on the outside of the cytoplasmic membrane and requires two reduced cysteinyls at the heme binding site. A constructed ResA-deficient Bacillus subtilis strain was found to lack c-type cytochromes. Cytochrome c synthesis was restored in the mutant by: (i) in trans expression of resA; (ii) deficiency in BdbD, a thiol-disulfide oxidoreductase that catalyzes formation of an intramolecular disulfide bond in apocytochrome c after transfer of the polypeptide across the cytoplasmic membrane; or (iii) by addition of the reductant dithiothreitol to the growth medium. In vivo studies of ResA showed that it is membrane-associated with its thioredoxin-like domain on the outside of the cytoplasmic membrane. Analysis of a soluble form of the protein revealed two redox reactive cysteine residues with a midpoint potential of about -340 mV at pH 7. We conclude that ResA, probably together with another thiol-disulfide oxidoreductase, CcdA, is required for the reduction of the cysteinyls in the heme binding site of apocytochrome c.
- Subjects :
- Amino Acid Sequence
Amino Acids chemistry
Bacterial Proteins metabolism
Base Sequence
Binding Sites
Cell Membrane metabolism
Chromatography, Gel
Cysteine chemistry
Cytochrome c Group chemistry
Cytochrome c Group metabolism
Cytoplasm metabolism
DNA metabolism
Disulfides
Electron Transport Complex IV metabolism
Electrophoresis, Polyacrylamide Gel
Hydrogen-Ion Concentration
Immunoblotting
Membrane Proteins metabolism
Models, Biological
Molecular Sequence Data
Mutation
Oxidation-Reduction
Peptides chemistry
Phenotype
Plasmids metabolism
Protein Binding
Protein Disulfide Reductase (Glutathione) metabolism
Protein Structure, Tertiary
Thioredoxins metabolism
Bacillus subtilis metabolism
Cytochrome c Group biosynthesis
Membrane Proteins chemistry
Membrane Proteins physiology
Protein Disulfide Reductase (Glutathione) chemistry
Protein Disulfide Reductase (Glutathione) physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9258
- Volume :
- 278
- Issue :
- 20
- Database :
- MEDLINE
- Journal :
- The Journal of biological chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 12637552
- Full Text :
- https://doi.org/10.1074/jbc.M300103200