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Cell-type-dependent repression of yeast a-specific genes requires Itc1p, a subunit of the Isw2p-Itc1p chromatin remodelling complex.
- Source :
-
Microbiology (Reading, England) [Microbiology (Reading)] 2003 Feb; Vol. 149 (Pt 2), pp. 341-351. - Publication Year :
- 2003
-
Abstract
- In Saccharomyces cerevisiae MATa haploid cells, the a-specific genes are expressed, whereas in the MATalpha haploid and MATa/alpha diploid cell types their transcription is repressed. It is shown in this report that Itc1p, a component of the ATP-dependent Isw2p-Itc1p chromatin remodelling complex, is required for the repression of a-specific genes. It has previously been reported that disruption of the ITC1 gene leads, in MATalpha cells, to an aberrant cell morphology resembling the polarized mating projection of cells responding to pheromone. The activation of the pheromone signalling pathway in itc1 mutants of both mating types was examined and found to be constitutively active in MATalpha itc1 but not in MATa itc1 cells. Furthermore, unlike the wild-type, MATalpha itc1 and MATa/alpha itc1/itc1 cells secrete a-factor and express significant levels of other a-specific genes. The results indicate that the inappropriate a-factor production in a MATalpha context, due to the derepression of the a-specific genes, produces an autocrine signalling loop that leads to the aberrant morphology displayed by MATalpha itc1 cells. It is suggested that the Isw2p-Itc1p complex contributes to maintain the repressive chromatin structure described for the asg operator present in the promoters of a-specific genes.
- Subjects :
- Adenosine Triphosphatases genetics
Adenosine Triphosphatases metabolism
Adenosine Triphosphate metabolism
Diploidy
Genes, Fungal
Haploidy
Homeodomain Proteins metabolism
Mutation
Pheromones pharmacology
Repressor Proteins metabolism
Saccharomyces cerevisiae physiology
Saccharomyces cerevisiae ultrastructure
Saccharomyces cerevisiae Proteins metabolism
Signal Transduction
Transcription Factors genetics
Transcription Factors metabolism
Chromatin metabolism
Gene Expression Regulation, Fungal
Homeodomain Proteins genetics
Repressor Proteins genetics
Saccharomyces cerevisiae genetics
Saccharomyces cerevisiae Proteins genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1350-0872
- Volume :
- 149
- Issue :
- Pt 2
- Database :
- MEDLINE
- Journal :
- Microbiology (Reading, England)
- Publication Type :
- Academic Journal
- Accession number :
- 12624196
- Full Text :
- https://doi.org/10.1099/mic.0.25920-0