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Diverse fibrillar peptides directly bind the Alzheimer's amyloid precursor protein and amyloid precursor-like protein 2 resulting in cellular accumulation.
- Source :
-
Brain research [Brain Res] 2003 Mar 21; Vol. 966 (2), pp. 231-44. - Publication Year :
- 2003
-
Abstract
- The Alzheimer's disease Abeta peptide can increase the levels of cell-associated amyloid precursor protein (APP) in vitro. To determine the specificity of this response for Abeta and whether it is related to cytotoxicity, we tested a diverse range of fibrillar peptides including amyloid-beta (Abeta), the fibrillar prion peptides PrP106-126 and PrP178-193 and human islet-cell amylin. All these peptides increased the levels of APP and amyloid precursor-like protein 2 (APLP2) in primary cultures of astrocytes and neurons. Specificity was shown by a lack of change to amyloid precursor-like protein 1, tau-1 and cellular prion protein (PrP(c)) levels. APP and APLP2 levels were elevated only in cultures exposed to fibrillar peptides as assessed by electron microscopy and not in cultures treated with non-fibrillogenic peptide variants or aggregated lipoprotein. We found that PrP106-126 and the non-toxic but fibril-forming PrP178-193 increased APP levels in cultures derived from both wild-type and PrP(c)-deficient mice indicating that fibrillar peptides up-regulate APP through a non-cytotoxic mechanism and irrespective of parental protein expression. Fibrillar PrP106-126 and Abeta peptides bound recombinant APP and APLP2 suggesting the accumulation of these proteins was mediated by direct binding to the fibrillated peptide. This was supported by decreased APP accumulation following extensive washing of the cultures to remove fibrillar aggregates. Pre-incubation of fibrillar peptide with recombinant APP18-146, the putative fibril binding site, also abrogated the accumulation of APP. These findings show that diverse fibrillogenic peptides can induce accumulation of APP and APLP2 and this mechanism could contribute to pathogenesis in neurodegenerative disorders.
- Subjects :
- Amino Acid Sequence
Amyloid beta-Peptides classification
Amyloid beta-Peptides pharmacology
Amyloid beta-Protein Precursor ultrastructure
Animals
Astrocytes drug effects
Astrocytes metabolism
Cell Survival physiology
Cells, Cultured
Humans
Immunoblotting methods
Mice
Mice, Inbred C57BL
Mice, Knockout
Microglia drug effects
Microglia metabolism
Microscopy, Electron methods
Nerve Tissue Proteins ultrastructure
Neurons drug effects
Neurons ultrastructure
Peptide Fragments pharmacology
Prions classification
Prions pharmacology
Protein Binding
Recombinant Proteins
Alzheimer Disease metabolism
Amyloid beta-Peptides metabolism
Amyloid beta-Protein Precursor metabolism
Nerve Tissue Proteins metabolism
Neurons metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0006-8993
- Volume :
- 966
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Brain research
- Publication Type :
- Academic Journal
- Accession number :
- 12618346
- Full Text :
- https://doi.org/10.1016/s0006-8993(02)04173-2