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Sleep modulates cannabinoid receptor 1 expression in the pons of rats.
- Source :
-
Neuroscience [Neuroscience] 2003; Vol. 117 (1), pp. 197-201. - Publication Year :
- 2003
-
Abstract
- Endocannabinoids seem to play a role in the modulation of alertness. Therefore, we measured cannabinoid receptor 1 (CB1R) protein by Western blot and messenger RNA (mRNA) by reverse transcription-polymerase chain reaction in the pons of rats across the 24-h period. We performed evaluations every 4 h beginning at 09:00 h. Rats were under a controlled light/dark cycle 12:12 (lights on at 08:00 h). Our data suggest that the expression of CB1R gene depends on diurnal variations, with maximum expression at 13:00 h for protein and 21:00 h for mRNA, and minimum expression at 01:00 and 09:00 h, respectively. We also analyzed CB1R protein and mRNA levels in the pons of rats deprived of total sleep for 24 h and in rats with a 24-h period of sleep deprivation plus a 2-h period of sleep rebound. Unlike sleep deprivation, sleep rebound significantly increased CB1R protein while decreasing mRNA. Despite the fact that we used gentle manipulation to deprive the animals of sleep, there may be a potential influence of stress on this effect, too. However, these facts suggest that CB1R gene expression is modulated by the light/dark cycle and by sleep.
- Subjects :
- Animals
Cannabinoid Receptor Modulators
Circadian Rhythm physiology
Gene Expression Regulation physiology
Male
RNA, Messenger biosynthesis
Rats
Rats, Wistar
Receptors, Cannabinoid
Sleep physiology
Fatty Acids, Unsaturated biosynthesis
Pons metabolism
Receptors, Drug biosynthesis
Sleep Deprivation metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0306-4522
- Volume :
- 117
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Neuroscience
- Publication Type :
- Academic Journal
- Accession number :
- 12605905
- Full Text :
- https://doi.org/10.1016/s0306-4522(02)00820-5