Back to Search
Start Over
Experimental protection of mice against lethal Staphylococcus aureus infection by novel bacteriophage phi MR11.
- Source :
-
The Journal of infectious diseases [J Infect Dis] 2003 Feb 15; Vol. 187 (4), pp. 613-24. Date of Electronic Publication: 2003 Feb 07. - Publication Year :
- 2003
-
Abstract
- The protective effects of bacteriophages were assessed against experimental Staphylococcus aureus infection in mice. Of the S. aureus phages isolated in the study, phi MR11 was representatively used for all testing, because its host range was the most broad and it carries no genes for known toxins or antibiotic resistance. Intraperitoneal injections (8 x 10(8) cells) of S. aureus, including methicillin-resistant bacteria, caused bacteremia and eventual death in mice. In contrast, subsequent intraperitoneal administration of purified phi MR11 (MOI > or = 0.1) suppressed S. aureus-induced lethality. This lifesaving effect coincided with the rapid appearance of phi MR11 in the circulation, which remained at substantial levels until the bacteria were eradicated. Inoculation with high-dose phi MR11 alone produced no adverse effects attributable to the phage. These results uphold the efficacy of phage therapy against pernicious S. aureus infections in humans and suggest that phi MR11 may be a potential prototype for gene-modified, advanced therapeutic S. aureus phages.
- Subjects :
- Animals
Bacteremia prevention & control
Disease Models, Animal
Female
Methicillin pharmacology
Methicillin Resistance
Mice
Mice, Inbred BALB C
Staphylococcal Infections therapy
Staphylococcal Infections prevention & control
Staphylococcus Phages isolation & purification
Staphylococcus aureus drug effects
Staphylococcus aureus virology
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1899
- Volume :
- 187
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- The Journal of infectious diseases
- Publication Type :
- Academic Journal
- Accession number :
- 12599078
- Full Text :
- https://doi.org/10.1086/374001