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Combined action of vasoactive amines and bradykinin mediates allergen-evoked thermal hyperalgesia in rats.
- Source :
-
European journal of pharmacology [Eur J Pharmacol] 2003 Feb 21; Vol. 462 (1-3), pp. 185-92. - Publication Year :
- 2003
-
Abstract
- The ability of allergens to induce hyperalgesia in immunoglobulin E (IgE)-sensitized rats was investigated. The left hind paws of Wistar rats were sensitized with intraplantar injections of IgE anti-dinitrophenylated bovine serum albumin monoclonal antibody, and challenged with dinitrophenylated bovine serum albumin 24 h later. Allergen challenge yielded rapid thermal hyperalgesia and oedema formation in the ipsilateral paws, both reaching a plateau from 15 min to 3 h, and both diminishing thereafter. Allergen-evoked hyperalgesia was inhibited by intraperitoneal treatment with meclizine or methysergide, histamine and 5-hydroxytryptamine receptor antagonists. There was also sensitivity to local treatment with either bradykinin B(1) or B(2) receptor antagonists, des-Arg(9)-[Leu(8)]-bradykinin or D-arginyl-[Hyp3, Thi5, D-Tic7, Oic8]-bradykinin (Hoe 140). Anaphylactic hyperalgesia was mimicked by the combined administration of histamine, 5-hydroxytryptamine and bradykinin at doses which were ineffective when injected alone. This synergistic effect was abolished by treatment with either meclizine, methysergide, Hoe 140 or des-Arg(9)-[Leu(8)]-bradykinin. Our findings show that local thermal hyperalgesia is a feature of allergen-evoked inflammation, and that a synergistic interaction among bradykinin, 5-hydroxytryptamine and histamine plays a critical role in this phenomenon.
- Subjects :
- Allergens immunology
Animals
Bradykinin pharmacology
Bradykinin Receptor Antagonists
Cyproheptadine pharmacology
Dinitrophenols metabolism
Dose-Response Relationship, Drug
Drug Synergism
Edema chemically induced
Edema prevention & control
Histamine H1 Antagonists pharmacology
Hyperalgesia immunology
Hyperalgesia prevention & control
Immunoglobulin E immunology
Male
Meclizine pharmacology
Methysergide pharmacology
Pain chemically induced
Pain immunology
Pain prevention & control
Rats
Rats, Wistar
Serotonin Antagonists pharmacology
Serum Albumin, Bovine administration & dosage
Serum Albumin, Bovine metabolism
Time Factors
Bradykinin administration & dosage
Bradykinin analogs & derivatives
Histamine administration & dosage
Hyperalgesia chemically induced
Serotonin administration & dosage
Subjects
Details
- Language :
- English
- ISSN :
- 0014-2999
- Volume :
- 462
- Issue :
- 1-3
- Database :
- MEDLINE
- Journal :
- European journal of pharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 12591112
- Full Text :
- https://doi.org/10.1016/s0014-2999(02)02947-3