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Rearrangement of immunoglobulin heavy and light chains and VH family in thyroid and salivary gland lymphomas.
- Source :
-
Pathology international [Pathol Int] 2002 Dec; Vol. 52 (12), pp. 747-54. - Publication Year :
- 2002
-
Abstract
- It is often difficult to differentiate extranodal marginal zone B-cell lymphoma of mucosa-associated lymphoid tissue (MALT lymphoma) from non-neoplastic inflammatory conditions. Demonstration of clonal lymphoid proliferation by molecular procedures is important for accurate diagnosis. We examined the clonal population of B-cell lymphomas in nine cases of thyroid and two cases of salivary gland B-cell lymphoma using semi-nested polymerase chain reaction (PCR)-based assay for IgH gene arrangement and reverse transcription (RT)-PCR single-strand conformation polymorphism (SSCP) for the detection of IgL gene rearrangement. Clonality was evident in nine out of 11 cases of B-cell lymphomas examined by PCR, and in six of eight cases by RT-PCR SSCP. In addition, analysis of VH families was performed in eight cases. Although VH3 family was frequently used, each case demonstrated the VH4, VH5 or VH6 family. It is possible that the normal counterpart of thyroid or salivary gland lymphoma might be different from peripheral blood B lymphocytes, which usually use VH3 family. Our results indicate that although no clonality was noted in one case by both PCR and SSCP, these molecular methods are useful as supplementary diagnostic tests for both thyroid and salivary gland lymphomas.
- Subjects :
- Adult
Aged
Aged, 80 and over
Clone Cells
DNA Primers chemistry
Female
Humans
Lymphoma, B-Cell, Marginal Zone pathology
Male
Middle Aged
Polymorphism, Single-Stranded Conformational
RNA, Neoplasm genetics
Reverse Transcriptase Polymerase Chain Reaction
Salivary Gland Neoplasms pathology
Thyroid Neoplasms pathology
Gene Rearrangement, B-Lymphocyte, Heavy Chain
Gene Rearrangement, B-Lymphocyte, Light Chain
Immunoglobulin Variable Region genetics
Lymphoma, B-Cell, Marginal Zone genetics
Salivary Gland Neoplasms genetics
Thyroid Neoplasms genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1320-5463
- Volume :
- 52
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Pathology international
- Publication Type :
- Academic Journal
- Accession number :
- 12588443
- Full Text :
- https://doi.org/10.1046/j.1440-1827.2002.01418.x