Cloning and characterization of a gene cluster for geldanamycin production in Streptomyces hygroscopicus NRRL 3602.

Authors :: Rascher A
Hu Z
Viswanathan N
Schirmer A
Reid R
Nierman WC
Lewis M
Hutchinson CR
Source :: FEMS microbiology letters [FEMS Microbiol Lett] 2003 Jan 28; Vol. 218 (2), pp. 223-30.
Publication Year :: 2003
Abstract: We illustrate the use of a PCR-based method by which the genomic DNA of a microorganism can be rapidly queried for the presence of type I modular polyketide synthase genes to clone and characterize, by sequence analysis and gene disruption, a major portion of the geldanamycin production gene cluster from Streptomyces hygroscopicus var. geldanus NRRL 3602.

Subjects :: Base Sequence
Benzoquinones
Cloning, Molecular
DNA, Bacterial chemistry
DNA, Bacterial classification
DNA, Bacterial genetics
Gene Expression Regulation, Bacterial
Lactams, Macrocyclic
Models, Molecular
Molecular Sequence Data
Quinones isolation & purification
Quinones pharmacology
Streptomyces metabolism
Genes, Bacterial
Multigene Family
Quinones metabolism
Streptomyces genetics

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