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Coding of sweet, bitter, and umami tastes: different receptor cells sharing similar signaling pathways.
- Source :
-
Cell [Cell] 2003 Feb 07; Vol. 112 (3), pp. 293-301. - Publication Year :
- 2003
-
Abstract
- Mammals can taste a wide repertoire of chemosensory stimuli. Two unrelated families of receptors (T1Rs and T2Rs) mediate responses to sweet, amino acids, and bitter compounds. Here, we demonstrate that knockouts of TRPM5, a taste TRP ion channel, or PLCbeta2, a phospholipase C selectively expressed in taste tissue, abolish sweet, amino acid, and bitter taste reception, but do not impact sour or salty tastes. Therefore, despite relying on different receptors, sweet, amino acid, and bitter transduction converge on common signaling molecules. Using PLCbeta2 taste-blind animals, we then examined a fundamental question in taste perception: how taste modalities are encoded at the cellular level. Mice engineered to rescue PLCbeta2 function exclusively in bitter-receptor expressing cells respond normally to bitter tastants but do not taste sweet or amino acid stimuli. Thus, bitter is encoded independently of sweet and amino acids, and taste receptor cells are not broadly tuned across these modalities.
- Subjects :
- Action Potentials drug effects
Action Potentials physiology
Animals
Cell Membrane drug effects
Cells, Cultured
Citric Acid pharmacology
Female
GTP-Binding Proteins drug effects
GTP-Binding Proteins metabolism
Glutamic Acid pharmacology
Isoenzymes genetics
Male
Membrane Proteins genetics
Mice
Mice, Knockout
Neurons, Afferent drug effects
Neurons, Afferent physiology
Phospholipase C beta
Quinine pharmacology
Receptors, Cell Surface drug effects
Sensory Receptor Cells drug effects
Sensory Receptor Cells physiology
Signal Transduction drug effects
Signal Transduction physiology
Sucrose pharmacology
TRPM Cation Channels
Taste drug effects
Taste Buds cytology
Taste Buds drug effects
Type C Phospholipases genetics
Cell Membrane metabolism
Isoenzymes deficiency
Membrane Proteins deficiency
Receptors, Cell Surface metabolism
Taste genetics
Taste Buds metabolism
Type C Phospholipases deficiency
Subjects
Details
- Language :
- English
- ISSN :
- 0092-8674
- Volume :
- 112
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Cell
- Publication Type :
- Academic Journal
- Accession number :
- 12581520
- Full Text :
- https://doi.org/10.1016/s0092-8674(03)00071-0