[Determination of T lymphocytes and trace elements in spleen from rats infected with Toxoplasma gondii].

Objective: To determine the level of five trace elements(Fe2+, Cu2+, Zn2+, Ca2+, Mg2+) in the spleen and changes of T lymphocyte and its subtype variations in peripheral blood from the rats infected with Toxoplasma gondii.
Methods: Twenty rats were randomly and equally divided into two groups: control group and experiment group. Each rat in the experiment group received an i.p. injection of 2 ml normal saline containing 1.5 x 10(6) tachyzoites of T. gondii. On the 64th day after injection of T. gondii, the changes in T lymphocytes (TL) and their subgroups, the helper T lymphocytes (Th) and the suppressor T lymphocytes(Ts) in the peripheral blood of the rats with T. gondii were determined by the assay of the lymphocytes labeled with intercellular acid alpha-naphthyl acetate esterase. All the rats were killed and the atomic absorption method were used for detecting the level of trace elements in the spleen tissue.
Results: The number of TL and Th in experiment group was significantly lower than that of control (P < 0.01, P < 0.01). The ratio of Th/Ts showed a significant difference between the two groups. The level of Fe2+, Cu2+ in experiment group was significantly reduced (P < 0.01). The amount of Mg2+ in infected rats was higher than that of the control(P < 0.01). No statistical difference in the content of Zn2+, Ca2+ was found between the two groups.
Conclusion: T. gondii infection might cause the changes in the TL and Th in peripheral blood and the changes in trace elements in spleen of the rats.