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Elevated expression of valosin-containing protein (p97) in hepatocellular carcinoma is correlated with increased incidence of tumor recurrence.
- Source :
-
Journal of clinical oncology : official journal of the American Society of Clinical Oncology [J Clin Oncol] 2003 Feb 01; Vol. 21 (3), pp. 447-52. - Publication Year :
- 2003
-
Abstract
- Purpose: Valosin-containing protein (VCP; also known as p97) has been shown to be associated with antiapoptotic function and metastasis via activation of the nuclear factor-kappaB signaling pathway. In this study, association of VCP expression with recurrence of hepatocellular carcinoma (HCC) and patient survival was examined.<br />Patients and Methods: VCP expression in 170 patients (139 male and 31 female) with ages ranging from 31 to 81 years (median, 61 years) was analyzed by quantitative reverse-transcription polymerase chain reaction (RT-PCR) and immunohistochemistry, in which staining intensity in tumor cells was categorized as weaker (level 1) or equal to or stronger (level 2) than that in endothelial cells.<br />Results: Immunohistochemically, 57 patients (35.2%) showed level 1, and 105 patients (64.8%) showed level 2, VCP expression. Quantitative RT-PCR analysis revealed higher VCP mRNA expression in level 2 patients (n = 7) than level 1 (n = 4) (P <.05). Patients with VCP-level 2 HCC showed higher rate of portal vein invasion in the tumor (P <.01) and poorer disease-free and overall survival (P <.0001 and P <.05, respectively) compared with level 1 patients. Multivariate analysis revealed VCP expression level, tumor multiplicity, and degree of fibrosis in the noncancerous liver tissue to be independent prognosticators for disease-free and overall survival. VCP level was an indicator for disease-free survival in each early- (I and II) and advanced- (III and IV) stage group of pathologic tumor-node-metastasis classification (P <.001 and P <.01, respectively).<br />Conclusion: VCP expression level has prognostic significance for disease-free and overall survival of patients with HCC.
- Subjects :
- Adenosine Triphosphatases
Adult
Aged
Aged, 80 and over
Carcinoma, Hepatocellular surgery
Disease-Free Survival
Female
Humans
Immunohistochemistry
Liver Cirrhosis
Liver Neoplasms surgery
Male
Middle Aged
Neoplasm Invasiveness
Neoplasm Metastasis
Predictive Value of Tests
Prognosis
Reverse Transcriptase Polymerase Chain Reaction
Valosin Containing Protein
Carcinoma, Hepatocellular genetics
Carcinoma, Hepatocellular pathology
Cell Cycle Proteins biosynthesis
Gene Expression Regulation, Neoplastic
Liver Neoplasms genetics
Liver Neoplasms pathology
Neoplasm Recurrence, Local
Subjects
Details
- Language :
- English
- ISSN :
- 0732-183X
- Volume :
- 21
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Journal of clinical oncology : official journal of the American Society of Clinical Oncology
- Publication Type :
- Academic Journal
- Accession number :
- 12560433
- Full Text :
- https://doi.org/10.1200/JCO.2003.06.068