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Identification of a homologue of a house dust mite allergen in a cDNA library from Sarcoptes scabiei var hominis and evaluation of its vaccine potential in a rabbit/S. scabiei var. canis model.
- Source :
-
The American journal of tropical medicine and hygiene [Am J Trop Med Hyg] 2003 Jan; Vol. 68 (1), pp. 54-60. - Publication Year :
- 2003
-
Abstract
- Sarcoptes scabiei ("itch mite") causes scabies, a disease of considerable human and veterinary significance. Little work has been done at the molecular level because of the difficulty of obtaining mites. We have used mites in skin from the bedding of crusted scabies patients for the construction of a library of 10(5) cDNAs from S. scabiei var. hominis cloned in the vector pGEX4T-2. We describe the isolation by immunoscreening of 2 clones, one of which (Ssagl) is homologous to and cross-reactive with the house dust mite Euroglyphus maynei allergen M-177, an apolipoprotein from hemolymph. Immunohistochemistry revealed that it is located around the internal organs and cuticle of the mite and in eggs. Although it was not found to be protective in a challenge trial, the rabbits did not exhibit typical crust characteristics. This work shows that it is now possible to conduct such challenge trials with cloned scabies antigens.
- Subjects :
- Allergens chemistry
Allergens genetics
Amino Acid Sequence
Animals
Antigens chemistry
Antigens genetics
Antigens immunology
Base Sequence
Cloning, Molecular
DNA, Complementary chemistry
DNA, Complementary genetics
Disease Models, Animal
Gene Library
Humans
Immune Sera immunology
Immunohistochemistry
Molecular Sequence Data
Polymerase Chain Reaction
Pyroglyphidae genetics
RNA, Messenger genetics
Rabbits
Sarcoptes scabiei genetics
Scabies immunology
Scabies parasitology
Sequence Alignment
Sequence Homology, Amino Acid
Allergens immunology
Pyroglyphidae immunology
Sarcoptes scabiei immunology
Scabies prevention & control
Vaccines genetics
Vaccines immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0002-9637
- Volume :
- 68
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- The American journal of tropical medicine and hygiene
- Publication Type :
- Academic Journal
- Accession number :
- 12556149