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Morphological analyses of interleukin-8 effects on rat ovarian follicles at ovulation and luteinization in vivo.
- Source :
-
Cytokine [Cytokine] 2002 Nov 24; Vol. 20 (4), pp. 168-73. - Publication Year :
- 2002
-
Abstract
- The aim of the present study was to elucidate functions of the interleukin (IL)-8 at ovulation and luteinization in vivo. To compare the morphological differences between human chorionic gonadotropin (hCG) and IL-8 stimulation, scanning electron microscopy was employed to study rat ovarian vascular corrosion casts. Follicular growth and increased capillary vessel densities around the follicles were seen in vascular corrosion casts after IL-8 injection, similar to the result of hCG administration. This result indicated that exogenous IL-8 could play a role in the neovascularization during follicular development as an angiogenetic factor. Many fenestrations were observed in the vascular endothelium by hCG administration. In contrast, no fenestrations were observed with IL-8 injection, indicating that IL-8 may not be sufficient to increase the vascular permeability directly. Although germinal vesicle breakdown (GVBD) occurred at rates of 82% after the hCG injection, only 20% GVBD was observed after the IL-8 injection. The present study indicated that IL-8 might have important effects on rat follicles at ovulation and luteinization via vascularization in a similar manner to hCG. However, IL-8 was not effective on vascular permeability and oocyte maturation, which were different from hCG. Thus, we can conclude that IL-8 can participate in follicular development in part and may play important roles in ovulation and luteinization as one of some mediators induced by endogenous luteinizing hormone.
- Subjects :
- Animals
Chorionic Gonadotropin pharmacology
Corrosion Casting
Endothelium, Vascular drug effects
Endothelium, Vascular ultrastructure
Female
Gonadotropins, Equine pharmacology
Humans
Luteinizing Hormone physiology
Microscopy, Electron, Scanning
Oocytes drug effects
Oogenesis drug effects
Ovarian Follicle blood supply
Ovarian Follicle ultrastructure
Ovulation Induction
Rats
Rats, Wistar
Interleukin-8 pharmacology
Luteinization drug effects
Ovarian Follicle drug effects
Ovulation drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1043-4666
- Volume :
- 20
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Cytokine
- Publication Type :
- Academic Journal
- Accession number :
- 12543081
- Full Text :
- https://doi.org/10.1006/cyto.2002.1987