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The Mad and Myc basic domains are functionally equivalent.
- Source :
-
The Journal of biological chemistry [J Biol Chem] 2003 Mar 28; Vol. 278 (13), pp. 11094-9. Date of Electronic Publication: 2003 Jan 21. - Publication Year :
- 2003
-
Abstract
- The Myc/Max/Mad family of transcription factors plays a fundamental role in the regulation of cell proliferation, oncogenic transformation, and cell differentiation. However, it remains unclear whether different heterodimers, such as Myc/Max and Mad/Max, recognize the same or different target genes in vivo. We show by chromatin immunoprecipitation that Myc target genes are also recognized by Mad1 in differentiated HL60 cells. We also substituted the complete basic region of Myc for the corresponding region of Mad. Wild-type c-Myc was then compared with c-Myc(Mad-BR) in oncogenic transformation, regulation of cell proliferation, induction of apoptosis, activation of chromosomal gene expression, and direct binding to chromosomal sites by chromatin immunoprecipitation. We find that the wild-type c-Myc and c-Myc/MadBR proteins have indistinguishable biological activity and target gene recognition in vivo. These data are consistent with a model in which Myc and Mad regulate a common set of target genes.
- Subjects :
- Amino Acid Sequence
Apoptosis physiology
Base Sequence
Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
DNA Primers
DNA-Binding Proteins chemistry
DNA-Binding Proteins metabolism
HL-60 Cells
Humans
Molecular Sequence Data
Protein Binding
Proto-Oncogene Proteins c-myc chemistry
Proto-Oncogene Proteins c-myc metabolism
DNA-Binding Proteins physiology
Proto-Oncogene Proteins c-myc physiology
Repressor Proteins
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9258
- Volume :
- 278
- Issue :
- 13
- Database :
- MEDLINE
- Journal :
- The Journal of biological chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 12538578
- Full Text :
- https://doi.org/10.1074/jbc.M212298200