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Activation of delta opioid receptors induces receptor insertion and neuropeptide secretion.
- Source :
-
Neuron [Neuron] 2003 Jan 09; Vol. 37 (1), pp. 121-33. - Publication Year :
- 2003
-
Abstract
- Here we describe a novel mechanism for plasma membrane insertion of the delta opioid receptor (DOR). In small dorsal root ganglion neurons, only low levels of DORs are present on the cell surface, in contrast to high levels of intracellular DORs mainly associated with vesicles containing calcitonin gene-related peptide (CGRP). Activation of surface DORs caused Ca(2+) release from IP(3)-sensitive stores and Ca(2+) entry, resulting in a slow and long-lasting exocytosis, DOR insertion, and CGRP release. In contrast, membrane depolarization or activation of vanilloid and P2Y(1) receptors induced a rapid DOR insertion. Thus, DOR activation induces a Ca(2+)-dependent insertion of DORs that is coupled to a release of excitatory neuropeptides, suggesting that treatment of inflammatory pain should include blockade of DORs.
- Subjects :
- Animals
Calcitonin Gene-Related Peptide metabolism
Calcium metabolism
Calcium Signaling drug effects
Calcium Signaling physiology
Cell Membrane drug effects
Cell Membrane ultrastructure
Exocytosis drug effects
Fluorescent Antibody Technique
Ganglia, Spinal drug effects
Ganglia, Spinal ultrastructure
Male
Membrane Potentials drug effects
Membrane Potentials physiology
Mice
Mice, Inbred C57BL
Microscopy, Electron
Neurons, Afferent drug effects
Neurons, Afferent ultrastructure
Neuropeptides metabolism
Nociceptors drug effects
Nociceptors ultrastructure
PC12 Cells
Pain metabolism
Pain physiopathology
Rats
Receptors, Drug drug effects
Receptors, Drug metabolism
Receptors, Neurotransmitter drug effects
Receptors, Neurotransmitter metabolism
Receptors, Opioid, delta drug effects
Receptors, Purinergic P2 drug effects
Receptors, Purinergic P2 metabolism
Receptors, Purinergic P2Y1
Secretory Vesicles metabolism
Secretory Vesicles ultrastructure
Cell Membrane metabolism
Exocytosis physiology
Ganglia, Spinal metabolism
Neurons, Afferent metabolism
Nociceptors metabolism
Receptors, Opioid, delta metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0896-6273
- Volume :
- 37
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Neuron
- Publication Type :
- Academic Journal
- Accession number :
- 12526778
- Full Text :
- https://doi.org/10.1016/s0896-6273(02)01103-0