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Dynamics of the early immune cellular reactions after myogenic cell transplantation.
- Source :
-
Cell transplantation [Cell Transplant] 2002; Vol. 11 (7), pp. 671-81. - Publication Year :
- 2002
-
Abstract
- The role of immune cells in the early donor cell death/survival following myoblast transplantation is confusing, one of the reasons being the lack of data about the immune reactions following cell transplantation. We used outbred mice as hosts for transplantation of primary cultured muscle cells and T-antigen-immortalized myoblasts. The host muscles were analyzed 1 h to 7 days after cell injection. No net loss of the donor primary cultured cell population was observed in this period. The immune cellular reaction in this case was: 1) a brief (<48 h) neutrophil invasion; 2) macrophage infiltration from days 1 to 7; 3) a specific response involving CTL and few NK cells (days 6 and 7), preceded by a low CD4+ cell infiltration starting at day 3. In contrast, donor-immortalized myoblasts completely disappeared during the 7-day follow-up. In this case, an intense infiltration of CTL and macrophages, with moderate CD4+ infiltration and lower amounts of NK cells, was observed starting at day 2. The nonspecific immune response at days 0 and 1 was similar for both types of donor cells. The present observations set a basis to interpret the role of immune cells on the early death/survival of donor cells following myoblast transplantation.
- Subjects :
- Animals
CD4-Positive T-Lymphocytes immunology
Cell Death immunology
Cell Line, Transformed immunology
Chemotaxis, Leukocyte immunology
Female
Killer Cells, Natural immunology
Macrophages immunology
Male
Mice
Muscle Cells immunology
Myoblasts immunology
Neutrophils immunology
T-Lymphocytes, Cytotoxic immunology
Cell Line, Transformed transplantation
Graft Survival immunology
Graft vs Host Reaction immunology
Muscle Cells transplantation
Myoblasts transplantation
Stem Cell Transplantation adverse effects
Subjects
Details
- Language :
- English
- ISSN :
- 0963-6897
- Volume :
- 11
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Cell transplantation
- Publication Type :
- Academic Journal
- Accession number :
- 12518894
- Full Text :
- https://doi.org/10.3727/000000002783985378