Back to Search
Start Over
Lipid raft distribution of CD4 depends on its palmitoylation and association with Lck, and evidence for CD4-induced lipid raft aggregation as an additional mechanism to enhance CD3 signaling.
- Source :
-
Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 2003 Jan 15; Vol. 170 (2), pp. 913-21. - Publication Year :
- 2003
-
Abstract
- By mutagenesis, we demonstrated that the palmitoylation of the membrane-proximal Cys(396) and Cys(399)of CD4, and the association of CD4 with Lck contribute to the enrichment of CD4 in lipid rafts. Ab cross-linking of CD4 induces an extensive membrane patching on the T cell surface, which is related to lipid raft aggregation. The lipid raft localization of CD4 is critical for CD4 to induce the aggregation of lipid rafts. The localization of CD4 in lipid rafts also correlates to the ability of CD4 to enhance receptor tyrosine phosphorylation. Thus, our data suggest that CD4-induced aggregation of lipid rafts may play an additional role in CD4 signaling besides its adhesion to MHC molecules and association with Lck.
- Subjects :
- Amino Acid Motifs genetics
Amino Acid Motifs immunology
Antibodies, Monoclonal metabolism
Binding Sites genetics
Binding Sites immunology
CD4 Antigens genetics
CD4 Antigens immunology
CD4 Antigens physiology
CD8 Antigens genetics
CD8 Antigens metabolism
Cell Membrane immunology
Cell Membrane metabolism
Cross-Linking Reagents metabolism
Cysteine genetics
Cysteine metabolism
Gene Targeting
Humans
Jurkat Cells
Lymphocyte Specific Protein Tyrosine Kinase p56(lck) genetics
Membrane Microdomains genetics
Muromonab-CD3 metabolism
Mutagenesis, Site-Directed
Phosphorylation
Plasmids immunology
Plasmids metabolism
Sequence Deletion immunology
Signal Transduction genetics
T-Lymphocytes immunology
T-Lymphocytes metabolism
Tyrosine metabolism
CD3 Complex physiology
CD4 Antigens metabolism
Lymphocyte Specific Protein Tyrosine Kinase p56(lck) metabolism
Membrane Microdomains metabolism
Palmitic Acid metabolism
Signal Transduction immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1767
- Volume :
- 170
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Journal of immunology (Baltimore, Md. : 1950)
- Publication Type :
- Academic Journal
- Accession number :
- 12517957
- Full Text :
- https://doi.org/10.4049/jimmunol.170.2.913