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A critical role for Syk protein tyrosine kinase in Fc receptor-mediated antigen presentation and induction of dendritic cell maturation.
- Source :
-
Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 2003 Jan 15; Vol. 170 (2), pp. 846-52. - Publication Year :
- 2003
-
Abstract
- Dendritic cells (DCs) are the only APCs capable of initiating adaptive immune responses. The initiation of immune responses requires that DCs 1) internalize and present Ags; and 2) undergo a differentiation process, called "maturation", which transforms DCs into efficient APCs. DC maturation may be initiated by the engagement of different surface receptors, including certain cytokine receptors (such as TNFR), Toll-like receptors, CD40, and FcRs. The early activation events that link receptor engagement and DC maturation are not well characterized. We found that FcR engagement by immune complexes induced the phosphorylation of Syk, a protein tyrosine kinase acting immediately downstream of FcRs. Syk was dispensable for DC differentiation in vitro and in vivo, but was strictly required for immune complexes internalization and subsequent Ag presentation to T lymphocytes. Importantly, Syk was also required for the induction of DC maturation and IL-12 production after FcR engagement, but not after engagement of other surface receptors, such as TNFR or Toll-like receptors. Therefore, protein tyrosine phosphorylation by Syk represents a novel pathway for the induction of DC maturation.
- Subjects :
- Animals
Bone Marrow Cells enzymology
Bone Marrow Cells immunology
Bone Marrow Cells metabolism
Cell Differentiation genetics
Cells, Cultured
Cytokines biosynthesis
Dendritic Cells immunology
Dendritic Cells metabolism
Enzyme Precursors deficiency
Enzyme Precursors genetics
Enzyme Precursors metabolism
Fetus
Interleukin Receptor Common gamma Subunit
Intracellular Signaling Peptides and Proteins
Liver Transplantation immunology
Mice
Mice, Inbred C57BL
Mice, Knockout
Phosphorylation
Protein-Tyrosine Kinases deficiency
Protein-Tyrosine Kinases genetics
Protein-Tyrosine Kinases metabolism
Receptors, IgG physiology
Receptors, Interleukin-7 deficiency
Receptors, Interleukin-7 genetics
Signal Transduction immunology
Syk Kinase
Transplantation Chimera genetics
Transplantation Chimera immunology
Antigen Presentation immunology
Cell Differentiation immunology
Dendritic Cells cytology
Dendritic Cells enzymology
Enzyme Precursors physiology
Protein-Tyrosine Kinases physiology
Receptors, Fc physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1767
- Volume :
- 170
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Journal of immunology (Baltimore, Md. : 1950)
- Publication Type :
- Academic Journal
- Accession number :
- 12517949
- Full Text :
- https://doi.org/10.4049/jimmunol.170.2.846