Back to Search
Start Over
Transepithelial transport of fluorescein in Caco-2 cell monolayers and use of such transport in in vitro evaluation of phenolic acid availability.
- Source :
-
Bioscience, biotechnology, and biochemistry [Biosci Biotechnol Biochem] 2002 Nov; Vol. 66 (11), pp. 2449-57. - Publication Year :
- 2002
-
Abstract
- Fluorescein is a marker-dye customary applied to the evaluation of tight-junctional permeability of epithelial cell monolayers. However, the true mechanism for the permeation has not been elucidated. Transepithelial transport of fluorescein in Caco-2 cell monolayers was therefore examined. Fluorescein transport was dependent on pH, and in a vectorical way in the apical-basolateral direction, but it was independent of the tight-junctional permeability of monolayers of these human intestinal cells. The permeation of fluorescein was concentration-dependent and saturable; the Michaelis constant was 7.7 mM and the maximum velocity was 40.3 nmol min(-1) (mg protein)(-1). Benzoic acid competitively inhibited fluorescein transport, suggesting that fluorescein is transported by a monocarboxylic acid transporter (MCT). Antioxidative polyphenolic compounds such as ferulic acid from dietary sources, competitively inhibited the permeation of fluorescein. These compounds probably share a transport carrier with fluorescein. Measurement of the effects of phenolic acids on fluorescein transport across Caco-2 monolayers would be a useful way to evaluate the intestinal absorption or bioavailability of dietary phenolic acids.
- Subjects :
- Benzoic Acid pharmacology
Biological Availability
Caco-2 Cells
Coumaric Acids pharmacology
Drug Interactions
Electric Impedance
Epithelial Cells metabolism
Humans
Hydrogen-Ion Concentration
Intestinal Absorption drug effects
Intestinal Mucosa cytology
Isoquinolines metabolism
Tight Junctions drug effects
Tight Junctions physiology
Fluorescein pharmacokinetics
Hydroxybenzoates pharmacokinetics
Intestinal Mucosa metabolism
Monocarboxylic Acid Transporters metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0916-8451
- Volume :
- 66
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Bioscience, biotechnology, and biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 12506986
- Full Text :
- https://doi.org/10.1271/bbb.66.2449