Back to Search
Start Over
Improved myocardial function with the addition of pinacidil to custadiol.
- Source :
-
Transplantation [Transplantation] 2002 Dec 27; Vol. 74 (12), pp. 1666-71. - Publication Year :
- 2002
-
Abstract
- Background: This study investigates the efficacy of pinacidil, an adenosine triphosphate-sensitive potassium (KATP) channel opening agent, added to custadiol solution on myocardial protection during deep hypothermia and prolonged global ischemia on isolated rat hearts.<br />Methods: After 20 minutes of stabilization, 24 rats were divided into two groups. In group I (n=12), hearts were arrested with cold (4 degrees C) custadiol solution containing 50 micromol/L of pinacidil and subsequently dipped into the same solution for 120 minutes at 4 degrees C. Group I hearts were perfused with low-flow pinacidil-custadiol (PC) solution during the ischemic period. Group II (n=12) hearts, after the stabilization period, were arrested with cold custadiol solution only, then subsequently dipped and perfused with the same solution for 120 minutes at 4 degrees C. All hearts were reperfused with Krebb's-Henseleit solution at 37 degrees C for 60 minutes. Hemodynamic parameters (peak systolic pressure, end diastolic pressure, maximum rate of increase of left ventricular pressure [+dP/dt], ischemic contracture, and coronary sinus flow) were recorded at the end of the stabilization period and at 10-minute intervals during the reperfusion period. Biochemical data (creatine kinase [CK-MB] washout and troponin I [cTnI] levels) were compared between the two groups.<br />Results: There was no significant difference in any hemodynamic or biochemical parameters between the two groups during the stabilization period. The peak systolic pressure, +dP/dt, ischemic contraction amplitude, and coronary flow values were significantly higher in group I ( P<0.05) compared with group II during reperfusion. End diastolic pressures as well as CK-MB and cTnI levels were lower in the pinacidil-treated group, which is consistent with improved functional recovery during the reperfusion period.<br />Conclusion: The addition of pinacidil to the preservation solution, custadiol, improves myocardial recovery after deep hypothermia and prolongs ischemia.
- Subjects :
- Animals
Blood Pressure
Coronary Circulation drug effects
Creatine Kinase analysis
Glucose pharmacology
Heart physiology
Hypothermia, Induced
Male
Mannitol pharmacology
Myocardial Contraction drug effects
Myocardium chemistry
Organ Preservation methods
Organ Preservation Solutions pharmacology
Potassium Chloride pharmacology
Procaine pharmacology
Rats
Rats, Sprague-Dawley
Troponin I analysis
Heart drug effects
Myocardial Ischemia physiopathology
Pinacidil pharmacology
Vasodilator Agents pharmacology
Ventricular Function, Left drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 0041-1337
- Volume :
- 74
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Transplantation
- Publication Type :
- Academic Journal
- Accession number :
- 12499877
- Full Text :
- https://doi.org/10.1097/00007890-200212270-00003