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[Effect of antisense oligonucleotide against telomerase in rat spermatogonia and its reactivity to cytokine].
- Source :
-
Zhonghua nan ke xue = National journal of andrology [Zhonghua Nan Ke Xue] 2002; Vol. 8 (5), pp. 319-22. - Publication Year :
- 2002
-
Abstract
- Objectives: To study the change of telomerase activity in rat spermatogonia when the telomerase RNA was enclosed, and reactivity of the change to cytokine(SCF, TGF-beta 1).<br />Methods: The antisense oligonucleotides(PS-ASON) of telomerase was transfected into proliferating spermatogonia in vitro with the liposomes as the vector. Then the cytokine, stem cell factor (SCF) or transforming growth factor-beta 1(TGF-beta 1), was added. The proliferative activity of the spermatogonia was determined before and after the inhibition by MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide]assay. The change of telomerase activity was detected by telomeric repeat amplification protocol (TRAP).<br />Results: 1 mumol/L PS-ASON obviously downregulated the telomerase activity and inhibited spermatogonia proliferation. When the inhibition was over, the activity recovered to some extent(P < 0.01). Growth factors can regulate the spermatogonia after inhibition, SCF may improve the activity of telomerase and the proliferation of spermatogonia. Adversely, TGF-beta 1 may inhibit the recovery of telomerase activity.<br />Conclusions: To inhibit spermatogonia telomerase activity antisensely can limit the proliferation of spermatogonia efficiently, which was regulated by cytokine. This method might be a new and efficient way in male birth control.
- Subjects :
- Animals
Bacterial Proteins analysis
Cell Division drug effects
Contraceptive Agents, Male pharmacology
Enzyme-Linked Immunosorbent Assay
Male
RNA-Binding Proteins analysis
Rats
Rats, Sprague-Dawley
Spermatogonia cytology
Spermatogonia enzymology
Stem Cell Factor metabolism
Telomerase genetics
Telomerase metabolism
Transcription Factors analysis
Transfection
Cytokines metabolism
Oligonucleotides, Antisense pharmacology
Spermatogonia drug effects
Telomerase antagonists & inhibitors
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1009-3591
- Volume :
- 8
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Zhonghua nan ke xue = National journal of andrology
- Publication Type :
- Academic Journal
- Accession number :
- 12479115